Multiple organic substrates support Mn(II) removal with enrichment of Mn(II)-oxidizing bacteria. (1st April 2020)

• Record Type: Journal Article
• Authors: Shoiful, Ahmad; Ohta, Taiki; Kambara, Hiromi; Matsushita, Shuji; Kindaichi, Tomonori; Ozaki, Noriatsu; Aoi, Yoshiteru; Imachi, Hiroyuki; Ohashi, Akiyoshi
• Is Part Of: Journal of environmental management. Volume 259(2020)
• Publication Date: 2020-04-01
• Subjects: Biological manganese oxidation -- Down-flow hanging sponge reactor -- K-medium -- Manganese-oxidizing bacteria -- Methanol -- Sterilized activated sludge; Environmental policy -- Periodicals; Environmental management -- Periodicals; Environment -- Periodicals; Ecology -- Periodicals; 363.705
• DOI: 10.1016/j.jenvman.2019.109771
• Languages: English
• ISSNs: 0301-4797

Abstract

Abstract: Three different organic substrates, K-medium, sterilized activated sludge (SAS), and methanol, were examined for utility as substrates for enriching manganese-oxidizing bacteria (MnOB) in an open bioreactor. The differences in Mn(II) oxidation performance between the substrates were investigated using three down-flow hanging sponge (DHS) reactors continuously treating artificial Mn(II)-containing water over 131 days. The results revealed that all three substrates were useful for enriching MnOB. Surprisingly, we observed only slight differences in Mn(II) removal between the substrates. The highest Mn(II) removal rate for the SAS-supplied reactor was 0.41 kg Mn⋅m −3 ⋅d −1, which was greater than that of K-medium, although the SAS performance was unstable. In contrast, the methanol-supplied reactor had more stable performance and the highest Mn(II) removal rate. We conclude that multiple genera of Comamonas, Pseudomonas, Mycobacterium, Nocardia and Hyphomicrobium play a role in Mn(II) oxidation and that their relative predominance was dependent on the substrate. Moreover, the initial inclusion of abiotic-MnO2 in the reactors promoted early MnOB enrichment. Graphical abstract: Image 1 Highlights: Manganese-oxidizing bacteria (MnOB) were enriched in an open continuous reactor. K-medium, sterilized activated sludge and methanol were promising substrates for MnOB. Abiotic manganese oxides were very effective for early Mn(II) removal performance. Pseudomonas, Mycobacterium, Nocardia and Hyphomicrobium likely play a role in Mn(II) oxidation.