Promotion of myotube differentiation and attenuation of muscle atrophy in murine C2C12 myoblast cells treated with teaghrelin. (5th January 2020)

• Record Type: Journal Article
• Authors: Hsieh, Sheng-Kuo; Lin, Hsin-Yi; Chen, Chun-Jung; Jhuo, Cian-Fen; Liao, Keng-Ying; Chen, Wen-Ying; Tzen, Jason T.C.
• Is Part Of: Chemico-biological interactions. Volume 315(2020)
• Publication Date: 2020-01-05
• Subjects: Chin-shin oolong -- Differentiation -- Ghrelin -- Muscle atrophy -- Teaghrelin; GHSR1a growth hormone secretagogue receptor 1a -- GH growth hormone -- AG acylated ghrelin -- G6, GHRP6 Growth hormone-releasing peptide 6 -- DMEM Dulbecco's modified Eagle medium -- FBS fetal bovine serum -- HS horse serum -- MHC myosin heavy chain -- MuRF1 muscle RING-finger protein-1 -- GM growth hormone -- DM differentiation medium -- TRITC tetramethylrhodamine -- IGF-1 insulin-like growth factor -- XTT (2, 3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide); Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2019.108893
• Languages: English
• ISSNs: 0009-2797

Abstract

Abstract: This study aimed to investigate the effects of teaghrelin, an active ingredient of Chin-shin oolong tea, on murine C2C12 myoblast cells. Under high serum conditions, teaghrelin inhibited C2C12 cell proliferation, indicating a cell cycle arrest and cessation of proliferative progression. Teaghrelin promoted pro-differentiation of C2C12 cells as evidenced by a progressively elongated morphology, as well as the induction of muscle specific myogenin, myosin heavy chain (MHC), and MyoD. The formation of multinucleated myotubes, and the increase of MHC-positive immunoreactivity within the myotubes, further reflected a complete differentiation and maturation of the contractile skeletal muscle cells induced by teaghrelin. Like ghrelin, teaghrelin attenuated dexamethasone-decreased myotube diameter, indicating its protective effects against skeletal muscle atrophy. Additionally, the expressions of Atrogin-1 and MuRF-1 ubiquitin E3 ligase were reduced. In conclusion, the results highlight a possibility of developing teaghrelin as a functional food for the prevention or therapeutic treatment of disease-associated skeletal muscle atrophy. Graphical abstract: Image 1 Highlights: Teaghrelin attenuated C2C12 myoblast cell proliferation. Teaghrelin promoted C2C12 cell differentiation and myotube formation. Teaghrelin attenuated dexamethasone-induced myotube atrophy. Teaghrelin attenuated dexamethasone-induced ubiquitin E3 ligase expression.