Receptor and signaling pathway involved in bovine lymphocyte activation by Atractylodis macrocephalae polysaccharides. (15th April 2020)

• Record Type: Journal Article
• Authors: Xu, Wei; Fang, Sijia; Wang, Yong; Chi, Xiaoqing; Ma, Xiaodan; Zhang, Tao; Hu, Songhua
• Is Part Of: Carbohydrate polymers. Volume 234(2020)
• Publication Date: 2020-04-15
• Subjects: Atractylodis macrocephalae polysaccharides -- Bovine lymphocytes -- Immunomodulation -- Receptor -- Signaling pathway -- miRNAs; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.115906
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: RAMPtp regulated cytokine, transcription factor and lymphocyte subpopulation. microRNAs in RAMPtp-stimulated SMLN lymphocytes were identified. RAMPtp enhanced immunocompetence of SMLN lymphocytes via multiple pathways. RAMPtp activated both T and B lymphocytes via binding to TCR and mIg individually. Abstract: The present study was to investigate the molecular mechanism underlying lymphocyte activation by total polysaccharides from Atractylodis macrocephalae (RAMPtp). The results showed that RAMPtp significantly promoted the secretions of cytokines (IFN-γ, IL-1α, IL-21, IFN-α, CCL4, CXCL9 and CXCL10), increased the proportions of CD4 + and CD8 + subpopulations, and enhanced the expressions of c-JUN, NFAT4, STAT1 and STAT3. microRNA sequencing identified 67 differentially expressed miRNAs (DEMs) in RAMPtp-stimulated SMLN lymphocytes, including 55 up-regulated and 12 down-regulated. GO and KEGG enrichment analyses of the predicted DEMs-targeted genes indicated that they were associated with immune system pathways, including PI3K-Akt, MAPKs, Jak-STAT and Calcium signaling pathways, which were confirmed by western blot and pathway inhibition assays. RAMPtp was further observed to favor immunostimulatory effect on both T and B lymphocytes via binding to TCR and membrane Ig individually. These findings might explain the immunomodulatory mechanism of RAMPtp in ameliorating the bovine intramammary infection.