Ionic liquids-modified cellulose coated magnetic nanoparticles for enzyme immobilization: Improvement of catalytic performance. (15th April 2020)

• Record Type: Journal Article
• Authors: Suo, Hongbo; Xu, Lili; Xue, Yu; Qiu, Xiang; Huang, He; Hu, Yi
• Is Part Of: Carbohydrate polymers. Volume 234(2020)
• Publication Date: 2020-04-15
• Subjects: Ionic liquids -- Covalent immobilization -- Magnetic cellulose nanoparticles -- Hydrophobicity -- Enzymatic performance; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.115914
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: Ionic liquids modified magnetic cellulose nanoparticles were synthesized. Enzymes covalently immobilized on IL-MCMC exhibited excellent activity and stability. The immobilized enzymes could be easily and effectively recovered. The prepared nanoparticles provided an excellent supports for enzyme immobilization. Abstract: In this work, ionic liquids-modified magnetic carboxymethyl cellulose nanoparticles (IL-MCMC) were prepared and used as supports for enzyme immobilization. The specific activity of immobilized lipase PPL-IL-MCMC was 1.43 and 2.81 folds higher than that of free PPL and PPL-MCMC, respectively. Water contact angle analysis indicated that the introduction of ionic liquids increased the hydrophobicity of supports, which in tune induced the lid-opening of lipase, allowing its active sites to become more accessible. In addition, the affinity between lipase and substrate immobilized on the prepared supports was enhanced. The same method was also applied to analyze immobilize penicillin G acylase (PGA) to further investigate the general applicability of the method. The results showed that the immobilized PGA exhibited higher stability than many other reported PGAs. The developed composites may be utilized as excellent supports for enzyme immobilization in industrial application.