Melatonin receptor protects cardiomyocyte against oxidative stress-induced apoptosis through the MAPK-ERK signaling pathway. (3rd March 2020)

• Record Type: Journal Article
• Authors: Li, Peng; Hu, Fang; Cao, Xin; Luo, Liyun; Tu, Qiuyun
• Is Part Of: Journal of receptor and signal transduction research. Volume 40:Number 2(2020)
• Page Start: 117
• Page End: 125
• Publication Date: 2020-03-03
• Subjects: Melatonin receptor -- cardiomyocyte -- ERK pathway -- oxidative stress; Cell receptors -- Periodicals; Cellular signal transduction -- Periodicals; 571.6
• DOI: 10.1080/10799893.2020.1719151
• Languages: English
• ISSNs: 1079-9893

Abstract

Abstract: Context: The role of melatonin receptor in cardiomyocyte oxidative stress has not been investigated. Aim: The aim of our study is to verify the beneficial effects of melatonin receptors on cardiomyocyte viability under oxidative stress. Methods: Cardiomyocytes were treated with hydrogen peroxide. Cell viability was measured via MTT assay and TUNEL staining. Then, anti-oxidative factors measurement and signaling pathway analysis were performed via qPCR and ELISA. Results: Melatonin receptor activation could attenuate hydrogen peroxide-mediated cardiomyocyte death via reducing apoptosis. At the molecular levels, melatonin receptor activation reduced inflammation response and maintained mitochondrial membrane potential. In addition, melatonin receptor activation is associated with decreased oxidative stress and increased anti-oxidative factors. Finally, we found that melatonin receptor activation triggered an elevation in the activity and expression of ERK pathway and blockade of ERK pathway would abolish the beneficial effects exerted by melatonin receptors activation on cardiomyocyte survival under oxidative stress. Conclusions: Our data suggest that melatonin receptor could attenuate oxidative stress injury in cardiomyocyte through regulation of the ERK signaling pathway.