1p36 is a chromosomal site of genomic instability in cervical intraepithelial neoplasia. (17th February 2020)

• Record Type: Journal Article
• Authors: Cortés-Gutiérrez, E. I.; García-Vielma, C.; Dávila-Rodríguez, M. I.; Sánchez-Dávila, H.; Fernández, J. L.; Gosálvez, J.
• Is Part Of: Biotechnic & histochemistry. Volume 95:Number 2(2020)
• Page Start: 137
• Page End: 144
• Publication Date: 2020-02-17
• Subjects: DNA Breakage detection-fluorescence in situ hybridization -- DBD–FISH -- cancer -- 1p36 chromosomal region -- cervical intraepithelial neoplasia; Histochemistry -- Periodicals; Stains and staining (Microscopy) -- Periodicals; Biotechnology -- Periodicals; 572
• DOI: 10.1080/10520295.2019.1652344
• Languages: English
• ISSNs: 1052-0295

Abstract

ABSTRACT: We investigated the association between progressive stages of cervical neoplasia and DNA damage in 1p36 DNA sequences of chromosome 1 in cervical epithelium using DNA breakage detection/fluorescence in situ hybridization (DBD-FISH). We used a hospital based unmatched case control study of 29 women that were grouped according to disease stage and selected according to histological diagnosis: 10 with low grade squamous intraepithelial lesions (LG-SILs), 10 with high grade SILs (HG-SILs) and nine with no cervical lesions; the 1pter sequence was used as internal control. We found a significant increase in the number of patients with HG-SIL compared to patients with LG-SILs or with no cervical lesions. 1p36 Genomic instability was validated by DBD-FISH using neutral comets. Genetic instability at specific gene loci, such as 1p36, might be characteristic of cervical cancer progression. DBD-FISH appears to be a useful approach for detecting and comparing damage to specific chromosomal regions related to the progression of cervical cancer.