Insights into the persistence and phenotypic effects of the endogenous and cryptic plasmid pMF1 in its host strain Myxococcus fulvus 124B02. Issue 3 (9th January 2020)

• Record Type: Journal Article
• Authors: Chen, Xiao-jing; Zhang, Zheng; Li, Ya-jie; Zhuo, Li; Sheng, Duo-hong; Li, Yue-zhong
• Is Part Of: FEMS microbiology ecology. Volume 96:Issue 3(2020)
• Publication Date: 2020-01-09
• Subjects: Myxococcus fulvus 124B02 -- endogenous plasmid -- persistent conditions -- pMF1 -- co-evolution -- long-term effects; Microbial ecology -- Periodicals; Microbiology -- Periodicals; 579.17
• DOI: 10.1093/femsec/fiaa001
• Languages: English
• ISSNs: 0168-6496

Abstract

ABSTRACT: Many endogenous plasmids carry no noticeable benefits for their bacterial hosts, and the persistence of these 'cryptic plasmids' and their functional impacts are mostly unclear. In this study, we investigated these uncertainties using the social bacterium Myxococcus fulvus 124B02 and its endogenous plasmid pMF1. pMF1 possesses diverse genes that originated from myxobacteria, suggesting a longstanding co-existence of the plasmid with various myxobacterial species. The curing of pMF1 from 124B02 had almost no phenotypic effects on the host. Laboratory evolution experiments showed that the 124B02 strain retained pMF1 when subcultured on dead Escherichia coli cells but lost pMF1 when subcultured on living E. coli cells or on casitone medium; these results indicated that the persistence of pMF1 in 124B02 was environment-dependent. Curing pMF1 caused the mutant to lose the ability to predate and develop fruiting bodies more quickly than the pMF1-containing strain after they were subcultured on dead E. coli cells, which indicated that the presence of pMF1 in M. fulvus 124B02 has some long-term effects on its host. The results provide some new insights into the persistence and impacts of cryptic plasmids in their natural bacterial cells. Abstract : This paper proposes a novel co-survival strategy of balanced heredity and physiology for the persistence of a cryptic plasmid in its host.