Immunization with the recombinant myosin regulatory light chain (FhrMRLC) in Adjuplex® adjuvant elicits a Th1-biased immune response and a reduction of parasite burden in Fasciola hepatica infected rats. (April 2020)

• Record Type: Journal Article
• Authors: Buffoni, L.; Piva, M.M.; Baska, P.; Januszkiewicz, K.; Norbury, L.J.; Prior, K.C.; Dezen, D.; Silva, A.S.; Wedrychowicz, H.; Mendes, R.E.
• Is Part Of: Parasitology international. Volume 75(2020)
• Publication Date: 2020-04
• Subjects: Immunization -- Vaccine -- Fasciola hepatica -- FhrMRLC; Parasitology -- Periodicals; Parasites -- Periodicals; Parasitic Diseases -- Periodicals; Parasitology -- Periodicals; Parasitologie -- Périodiques; 571.99905
• DOI: 10.1016/j.parint.2019.102037
• Languages: English
• ISSNs: 1383-5769

Abstract

Abstract: The aim of this study was to assess the immune response and the protective efficacy elicited by the vaccination with the recombinant Fasciola hepatica myosin regulatory light chain (FhrMRLC) in Adjuplex® adjuvant against the infection with F. hepatica in rats. Four groups of 15 animals each were used for the study, one group was immunized with the recombinant F. hepatica MRLC in Adjuplex® adjuvant and the other groups remained as adjuvant, positive and negative control groups. The parasitological study showed that a statistically significant reduction of 65.1% and 82.1% in fluke burden and fecal egg count, respectively, was detected in vaccinated animals. In addition, vaccination with FhrMRLC induced a well-defined humoral and cellular immune response characterized by a significant production of specific IgG and IL-2, IL-12, TNF-α and IFN-γ; which confirms the immunogenic capacity of the FhrMRLC. Graphical abstract: Unlabelled Image Highlights: A new vaccine candidate was assessed against infection with F. hepatica in rats. Fluke burden, hepatic damage and humoral and cellular immune response was analyzed. Vaccination elicited production of antibodies and Th1 cytokines in vaccinated group. A significant reduction of the fluke burden and fecal egg count was obtained.