Miniaturized culture method for the statistical study of growth rate and carbohydrate content of Picochlorum sp. D8 isolated from the Persian Gulf. (April 2020)

• Record Type: Journal Article
• Authors: Olia, Mahroo Seyed Jafari; Azin, Mehrdad; Sepahi, Abbas Akhavan; Moazami, Nasrin
• Is Part Of: Renewable energy. Volume 149(2020)
• Page Start: 479
• Page End: 488
• Publication Date: 2020-04
• Subjects: Microplate -- Microalgae -- Plackett–Burman Design -- Carbohydrate production -- Central Composite Design; Renewable energy sources -- Periodicals; Power resources -- Periodicals; Énergies renouvelables -- Périodiques; Ressources énergétiques -- Périodiques; 333.794
• DOI: 10.1016/j.renene.2019.12.069
• Languages: English
• ISSNs: 0960-1481

Abstract

Abstract: The Microplate-based method is a useful means for high-throughput screening and kinetic studies of microalgal growth. In this study, the statistical evaluation of growth and carbohydrate production by a marine microalga, designated as Picochlorum sp. D8, which is taxonomically affiliated to Picochlorum sp. SENEW3 was done on a miniaturized scale. Initially, the significance of various factors was studied by using the Plackett–Burman Design. The Central Composite Design was applied to compute the optimum level of significant factors, e.g., phosphate (KH2 PO4 +K2 HPO4 ) and FeCl3 ·6H2 O. Maximum carbohydrate production was obtained at low phosphate (0.148 g/L) and FeCl3 ·6H2 O (0.009 g/L) concentrations. The validation experiments were carried out under optimal conditions, where carbohydrate content increased by 3.5 times. The analyses of the results showed a quadratic effect of phosphate and FeCl3 ·6H2 O concentrations. Highlights: Introducing a new microalga for the production of carbohydrate and bioethanol. Describing the microplate-based method as an effective platform for optimization. Phosphate and FeCl3 ·6H2 O significantly affect carbohydrate production. Under optimized condition, the carbohydrate content was increased by 3.5 folds.