CRISPR-Based Directed Evolution for Crop Improvement. Issue 3 (March 2020)

• Record Type: Journal Article
• Authors: Butt, Haroon; Zaidi, Syed Shan-e-Ali; Hassan, Norhan; Mahfouz, Magdy
• Is Part Of: Trends in biotechnology. Volume 38:Issue 3(2020)
• Page Start: 236
• Page End: 240
• Publication Date: 2020-03
• Subjects: CRISPR-Cas9 -- CRISPR-directed evolution -- crop breeding -- HDR -- NHEJ -- trait engineering; Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Genetic engineering -- Periodicals; Industrial microbiology -- Periodicals; 660.605
• DOI: 10.1016/j.tibtech.2019.08.001
• Languages: English
• ISSNs: 0167-7799

Abstract

Abstract : Directed evolution involves generating diverse sequence variants of a gene of interest to produce a desirable trait under selective pressure. CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9) systems can be programmed to target any genomic locus and perform targeted directed evolution. Here, we discuss the opportunities and challenges of this emerging platform for targeted crop improvement.