Functional MRI of the mouse olfactory system. (21st June 2019)

• Record Type: Journal Article
• Authors: Muir, Eric R.; Biju, K.C.; Cong, Linlin; Rogers, William E.; Torres Hernandez, Enrique; Duong, Timothy Q.; Clark, Robert A.
• Is Part Of: Neuroscience letters. Volume 704(2019)
• Page Start: 57
• Page End: 61
• Publication Date: 2019-06-21
• Subjects: ACAv ventral part of anterior cingulate area -- ACB nucleus accumbens -- AOB accessory olfactory bulb -- AON anterior olfactory nucleus -- BOLD blood oxygen level-dependent -- COAa cortical amygdalar area anterior part -- COApl cortical amygdalar area posterior part -- CP caudoputamen -- DG dentate gyrus -- ECT ectorhinal area -- Ent entorhinal cortex -- EPd endopiriform nucleus -- fMRI functional magnetic resonance imaging -- GL glomerular layer -- HIP hippocampus -- HY hypothalamus -- IAA isoamyl acetate -- IP intraperitoneal -- IV intravenous -- MB midbrain -- MION monocrystalline iron oxide nanoparticles -- MOp primary motor area -- MOs secondary motor area -- NLOT nucleus of the lateral olfactory tract -- ORBl lateral orbital area -- OT olfactory tubercle -- PERI perirhinal area -- Pir piriform cortex -- PL/ILA prelimbic and infralimbic cortices -- RSP retrosplenial area -- SSp primary somatosensory area -- TEa temporal association area -- TH thalamus -- THd dorsal thalamic areas -- TTd dorsal taenia tecta; Olfaction -- Olfactory bulb -- Olfactory cortex -- Odors -- Pheromone -- fMRI; Neurology -- Periodicals; Neurology -- Periodicals; Research -- Periodicals; Neurologie -- Périodiques; Neuroanatomie -- Périodiques; Neuropharmacologie -- Périodiques; Neurophysiologie -- Périodiques; Neurology; Periodicals; Electronic journals; 617.48
• DOI: 10.1016/j.neulet.2019.03.055
• Languages: English
• ISSNs: 0304-3940

Abstract

Highlights: Neural activation pattern throughout the mouse olfactory network was mapped for the first time. Spatial pattern of activation was odorant-specific. 11.7 T magnet provided high signal-to-noise ratio and resolution for imaging mouse olfactory system. Abstract: Although olfactory dysfunction is an early warning sign of Alzheimer's and Parkinson's diseases, and is commonly present in a range of other neurodegenerative disorders, the mechanisms for its pathogenesis are not yet clear. Since fMRI allows the mapping of spatial and temporal patterns of activity in multiple brain regions simultaneously, it serves as a powerful tool to study olfactory dysfunction in animal models of neurodegenerative diseases. Nonetheless, there have been no reports to date of mapping odor-induced activation patterns beyond the olfactory bulb to the extended networks of olfactory and limbic archicortex, likely due to the small size of the mouse brain. Therefore, using an 11.7 T magnet and a blood volume-weighted fMRI technique, we mapped the functional neuroanatomy of the mouse olfactory system. Consistent with reports on imaging of the much larger human brain, we mapped activity in regions of the olfactory bulb, as well as olfactory and limbic archicortex. By using two distinct odorants, we further demonstrated odorant-specific activation patterns. Our work thus provides a methodological framework for fMRI studies of olfactory dysfunction in mouse models of neurodegeneration.