Nutrient‐sensitive transcription factors TFEB and TFE3 couple autophagy and metabolism to the peripheral clock. (24th May 2019)

• Record Type: Journal Article
• Authors: Pastore, Nunzia; Vainshtein, Anna; Herz, Niculin J; Huynh, Tuong; Brunetti, Lorenzo; Klisch, Tiemo J; Mutarelli, Margherita; Annunziata, Patrizia; Kinouchi, Kenichiro; Brunetti‐Pierri, Nicola; Sassone‐Corsi, Paolo; Ballabio, Andrea
• Is Part Of: EMBO journal. Volume 38:Number 12(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-05-24
• Subjects: circadian rhythm -- gene oscillation -- MiT‐TFE -- REV‐ERBα; Molecular biology -- Periodicals; 572.805
• DOI: 10.15252/embj.2018101347
• Languages: English
• ISSNs: 0261-4189

Abstract

Abstract: Autophagy and energy metabolism are known to follow a circadian pattern. However, it is unclear whether autophagy and the circadian clock are coordinated by common control mechanisms. Here, we show that the oscillation of autophagy genes is dependent on the nutrient‐sensitive activation of TFEB and TFE3, key regulators of autophagy, lysosomal biogenesis, and cell homeostasis. TFEB and TFE3 display a circadian activation over the 24‐h cycle and are responsible for the rhythmic induction of genes involved in autophagy during the light phase. Genetic ablation of TFEB and TFE3 in mice results in deregulated autophagy over the diurnal cycle and altered gene expression causing abnormal circadian wheel‐running behavior. In addition, TFEB and TFE3 directly regulate the expression of Rev‐erbα ( Nr1d1 ), a transcriptional repressor component of the core clock machinery also involved in the regulation of whole‐body metabolism and autophagy. Comparative analysis of the cistromes of TFEB/TFE3 and REV‐ERBα showed an extensive overlap of their binding sites, particularly in genes involved in autophagy and metabolic functions. These data reveal a direct link between nutrient and clock‐dependent regulation of gene expression shedding a new light on the crosstalk between autophagy, metabolism, and circadian cycles. Synopsis: Autophagy and energy metabolism are regulated by nutrients and follow the circadian rhythm, but it is unclear whether common control mechanisms coordinate autophagy and the circadian clock. The crosstalk between TFEB/TFE3 transcription factors and REV‐ERBα repressor contributes to the circadian oscillation of autophagy. TFEB and TFE3 show a rhythmic activation over the 24‐h cycle. TFEB/TFE3 knockout mice display altered circadian behaviour. Transcriptional oscillation of autophagy genes is impaired in the liver and muscle of TFEB/TFE3 knockout mice. TFEB and TFE3 regulate the expression of clock machinery component REV‐ERBα in a clock‐independent manner. Transcriptional repression and induction by REV‐ERBα and TFEB/TFE3, respectively, contribute to the rhythmic expression of autophagy genes and to the circadian oscillation of autophagy. Abstract : Crosstalk between TFEB/TFE3 transcription factors and REV‐ERBα repressor contributes to the circadian oscillation of autophagy.