Noncontiguous finished genome sequence and description of Raoultibacter massiliensis gen. nov., sp. nov. and Raoultibacter timonensis sp. nov, two new bacterial species isolated from the human gut. Issue 6 (30th January 2019)

• Record Type: Journal Article
• Authors: Traore, Sory Ibrahima; Bilen, Melhem; Beye, Mamadou; Diop, Awa; Mbogning Fonkou, Maxime Descartes; Tall, Mamadou Lamine; Michelle, Caroline; Yasir, Muhammad; Ibraheem Azhar, Esam; Bibi, Fehmida; Bittar, Fadi; Jiman‐Fatani, Asif Ahmad; Daoud, Ziad; Cadoret, Fréderic; Fournier, Pierre‐Edouard; Edouard, Sophie
• Is Part Of: MicrobiologyOpen. Volume 8:Issue 6(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-01-30
• Subjects: culturomics -- human gut microbiota -- new bacterial species -- Raoultibacter massiliensis -- Raoultibacter timonensis -- taxonogenomics; Microbiology -- Periodicals; 579
• DOI: 10.1002/mbo3.758
• Languages: English
• ISSNs: 2045-8827

Abstract

Abstract: As part of the culturomics project aiming at describing the human microbiota, we report in this study the description of the new bacterial genus Raoultibacter gen. nov. that includes two new species, that is, R. massiliensis sp. nov. and R. timonensis sp. nov. The R. massiliensis type strain Marseille‐P2849 T was isolated from the fecal specimen of a healthy 19‐year‐old Saudi Bedouin, while R. timonensis type strain Marseille‐P3277 T was isolated from the feces of an 11‐year‐old pygmy female living in Congo. Strain Marseille‐P2849 T exhibited 91.4% 16S rRNA sequence similarity with Gordonibacter urolithinfaciens, its phylogenetic closest neighbor with standing in nomenclature. As well, strain Marseille‐P3277 T exhibited 97.96% 16S rRNA similarity with strain Marseille‐P2849 T . Both strains were Gram‐positive, motile, nonspore‐forming rod and form transparent microcolonies on blood agar in both anaerobic and microaerophilic atmospheres. The genome sizes of strain Marseille‐P2849 T and strain Marseille‐P3277 T were 3, 657, 161 bp and 4, 000, 215 bp, respectively. Using a taxono‐genomic approach combining the phenotypic, biochemical, and genomic characteristics, we propose the genus Raoultibacter gen. nov., which contains strains Marseille‐P2849 T (= CSUR P2849 T, = DSM 103407 T ) and Marseille‐P3277 T (=CCUG 70680 T, =CSUR P3277 T ) as type strains of the species R. massiliensis sp. nov., and R. timonensis sp. nov., respectively. Abstract : We report in this study the description of the new bacterial genus Raoultibacter gen. nov. that includes two new species, that is, R. massiliensis sp. nov. and R. timonensis sp. nov. The R. massiliensis type strain Marseille‐P2849T was isolated from the fecal specimen of a healthy 19‐year‐old Saudi Bedouin, while R. timonensis type strain Marseille‐P3277T was isolated from the feces of an 11‐year‐old pygmy female living in Congo. These novel Gram‐negative, motile, nonsporeforming coccobacilli form transparent microcolonies on blood agar in both anaerobic and microaerophilic atmospheres and belong to the family Eggerthellaceae. The genome sizes of these strains were 3, 657, 161 bp and 4, 000, 215 bp, and their G+C contents were 59.02 and 59.9 mol%, respectively.