Characteristics of crude oil‐degrading bacteria Gordonia iterans isolated from marine coastal in Taean sediment. Issue 6 (19th October 2018)

• Record Type: Journal Article
• Authors: Kim, Hyun‐Sook; Dong, Ke; Kim, Jinsoo; Lee, Sang‐Seob
• Is Part Of: MicrobiologyOpen. Volume 8:Issue 6(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-10-19
• Subjects: alkB gene -- Gordonia iterans Co17 -- marine and costal ecosystem -- petroleum contamination biodegradation -- whole genome de novo sequencing; Microbiology -- Periodicals; 579
• DOI: 10.1002/mbo3.754
• Languages: English
• ISSNs: 2045-8827

Abstract

Abstract: Crude oil is a major pollutant of marine and coastal ecosystems, and it causes environmental problems more seriously. It is believed ultimate and complete degradation is accomplished mainly by microorganisms. In this study, we aim to search out for bacterial strains with high ability in degrading crude oil. From sediments contaminated by the petroleum spilled in 2007, an accident in Taean, South Korea, we isolated thirty‐one bacterial strains in total with potential application in crude oil contamination remediation. In terms of removal percentage after 7 days, one of the strains, Co17, showed the highest removal efficiency with 84.2% of crude oil in Bushnell‐Haas media. The Co17 strain even exhibited outstanding ability removing crude oil at a high salt concentration. Through the whole genome sequencing annotation results, many genes related with n ‐alkane degradation in the genome of Gordonia sp. Co17, revealed alkane‐1‐monooxygenase, alcohol dehydrogenase, and Baeyer–Villiger monooxygenase. Specially, for confirmation of gene‐level, alk B gene encoding alkane hydroxylase (alkane‐1‐monooxygenase) was found in the strain Co17. The expression of alk B upregulated 125‐fold after 18 hr accompany with the removal of n ‐alkanes of 48.9%. We therefore propose the strain Gordonia iterans Co17, isolated from crude oil‐contaminated marine sediment, could be used to offer a new strategy for bioremediation with high efficiency. Abstract : From sediments contaminated by the petroleum spill in 2007, an accident in Taean, South Korea, we isolated 31 bacterial strains in total with potential application in petroleum contamination remediation. A strain Gordonia iterans Co17 has high ability in degrading crude oil under extreme environments and revealed the expression pattern of the related gene alkane hydroxylase ( alk B).