Expression Analysis of the Circular RNA Molecules in the Human Retinal Cells Treated with Homocysteine. (4th March 2019)

• Record Type: Journal Article
• Authors: Singh, Mahavir; George, Akash K; Homme, Rubens Petit; Majumder, Avisek; Laha, Anwesha; Sandhu, Harpal S.; Tyagi, Suresh C
• Is Part Of: Current eye research. Volume 44:Number 3(2019)
• Page Start: 287
• Page End: 293
• Publication Date: 2019-03-04
• Subjects: CircRNA -- epigenetics -- eye diseases -- inflammation -- retina; Ophthalmology -- Periodicals; Eye -- Diseases -- Periodicals; Ophthalmology -- Periodicals; 573.88
• DOI: 10.1080/02713683.2018.1542005
• Languages: English
• ISSNs: 0271-3683

Abstract

ABSTRACT: Purpose : To characterize the global profile of circular RNAs (circRNAs) and their differential expression levels in homocysteine (Hcy)-treated ARPE-19 cells, a line of human retinal pigment epithelial (RPE) cells. Materials and Methods : We treated ARPE-19 cells with and without Hcy to investigate the influence of Hcy on circRNA expression levels using dedicated human circRNA microarrays. Results : A total of 12, 233 circRNAs were identified out of them 54 were differentially expressed (17 were down-regulated, and 37 were up-regulated) with a fold change >2.0 ( p < 0.05) in Hcy-treated versus untreated cells. Conclusions : To our knowledge, this is the first report profiling circRNAs in human RPE cells post-Hcy treatment mimicking hyperhomocysteinemic (HHcy) conditions that negatively affect retinal biology and vision. These findings are of potential clinical significance as they will help understand Hcy metabolism and HHcy-mediated diseases and identify potential diagnostic and therapeutic targets for eye diseases that are caused by elevated Hcy concentrations.