Induced production of zinniol analogues by co-cultivation of two endophytic fungi in the same ecological niche. (February 2020)

• Record Type: Journal Article
• Authors: Wang, Zi-Ru; Yang, Hong-Xia; Peng, Xiao-Ping; Li, Gang; Lou, Hong-Xiang
• Is Part Of: Phytochemistry letters. Volume 35(2020)
• Page Start: 206
• Page End: 210
• Publication Date: 2020-02
• Subjects: Endophytes -- Co-cultivation -- Secondary metabolites -- Zinniol analogues -- Bioactivity; Botanical chemistry -- Periodicals; Chimie végétale -- Périodiques; 572.205
• DOI: 10.1016/j.phytol.2019.12.007
• Languages: English
• ISSNs: 1874-3900

Abstract

Graphical abstract: Highlights: Two fungal endophytes from the same plant tissue were subjected to co-cultivation. Three new zinniol analogs were isolated from the co-culture. The structures of compounds were established by HRMS, NMR, and optical options. Abstract: Three zinniol analogues, pleoniols A–C (1 –3 ), together with a known compound (4 ), were isolated from a mixed fermentation of two endophytic fungi, Pleosporales sp. F46 and Acremonium pilosum F47, both of which are found the pedicel of the medicinal plant Mahonia fortunei . High-performance liquid chromatography analysis showed that none of compounds 1 –4 were detected when either one of the two fungi was cultured alone. The structures of compounds 1 –4, including the absolute configurations, were deduced based on the interpretation of HRMS, NMR, and optical rotation data. Compounds 1 –3 exhibited no antimicrobial efficacies against four Gram-positive or Gram-negative bacteria and two plant pathogenic fungi. Moreover, they demonstrated no obvious cytotoxic activities against four cancer cell lines, A549, MDA-MB-231, CT-26 and MCF-7. Our work indicates that the co-culture of endophytes in the same ecological niche is an effective approach for promoting interspecies interaction and enriching chemical diversity.