Novel neuroprotective tetramethylpyrazine analog T-006 promotes neurogenesis and neurological restoration in a rat model of stroke. Issue 9 (12th June 2019)

• Record Type: Journal Article
• Authors: Wu, Liangmiao; Cao, Jie; Li, Ning; Chen, Haiyun; Liu, Wei; Zhang, Gaoxiao; Liu, Yu; Wang, Yuqiang; Sun, Yewei; Zhang, Zaijun
• Is Part Of: NeuroReport. Volume 30:Issue 9(2019)
• Publication Date: 2019-06-12
• Subjects: brain-derived neurotrophic factor -- ischemic stroke -- neurogenesis -- tetramethylpyrazine analog; Neurosciences -- Periodicals; Nervous system -- Periodicals; Neurophysiology -- Periodicals; Nervous System Diseases -- Periodicals; Nervous System Physiological Phenomena -- Periodicals; Neurosciences -- Periodicals; 616.805
• DOI: 10.1097/WNR.0000000000001256
• Languages: English
• ISSNs: 0959-4965

Abstract

Abstract : Neuronal death is among the deleterious pathological changes that occur after cerebral ischemia and can lead to transient or permanent neurological deficits. The tetramethylpyrazine analog T-006 has been shown to be a multifunctional neuroprotective agent; however, its neuroprotective effect and mechanism of action have not been studied in ischemic stroke model rats. This study investigated the neuroprotective effects of T-006 in rat stroke model using a battery of behavioral and molecular biological tests. Results indicated that T-006 treatment significantly improved neurological function and behavior. Double immunofluorescence staining showed that T-006 visibly improved the number of NeuN + /BrdU +, Nestin + /BrdU +, and DCX + /BrdU + cells and induced neuronal regeneration. Western blot analyses indicated that T-006 upregulated neurogenesis-related protein expression of postsynaptic density protein 95, brain-derived neurotrophic factor, synaptophysin, and myelin basic protein. Collectively, these data suggest that T-006 stimulated neurogenesis in rats with middle cerebral artery occlusion and restored neurological functions.