The Effect of Chyle Fat Injection on Human Hypertrophic Scars in an Animal Model: A New Strategy for the Treatment of Hypertrophic Scars. Issue 6 (June 2019)

• Record Type: Journal Article
• Authors: Chen, Junnan; Lai, Linying; Ma, Kui; Xu, Xiao; Huang, Zhenya; Zhou, Guiwen; Zhou, Yunchao; Liang, Liming; Chen, Minliang
• Is Part Of: Annals of plastic surgery. Volume 82:Issue 6(2019)
• Publication Date: 2019-06
• Subjects: chyle fat -- fat graft -- model -- scar; Surgery, Plastic -- Periodicals; 617.95205
• DOI: 10.1097/SAP.0000000000001784
• Languages: English
• ISSNs: 0148-7043

Abstract

Abstract : Background: Chyle fat transplantation has shown positive effects on preexisting human hypertrophic scars (HSs) in a nude mouse HS graft model. Methods: Hypertrophic scar fragments were obtained from 5 surgically treated burn patients and implanted into the backs of nude mice in 3 groups: group A, control; group B, triamcinolone; and group C, chyle fat. The specimens were implanted after the corresponding intralesional injection in each group, and the mice were observed for 4 weeks. In total, 18 mice and 72 scar specimens were studied. After 4 weeks, the HSs were removed from the mice. Then, the scar weights, histology, and decorin staining were assessed to evaluate the therapeutic efficacy. Results: An obviously significant difference was observed in the HS weight reduction between groups A and C ( P < 0.01), and a significant difference in the HS weight reduction was observed between groups A and B ( P < 0.05). However, there was no significant difference between groups B and C. The treatment groups (groups B and C) showed strong decorin staining. Furthermore, the decorin staining was much stronger in group C than in group B ( P < 0.05). Significant differences in extracellular matrix deposition were observed among the 3 groups, as determined by Masson trichrome staining. Both groups B and C showed significant therapeutic efficacy compared with group A, and group C exhibited a significant therapeutic effect compared with group B ( P < 0.05). Conclusions: This study indicates that chyle fat grafting is beneficial for treating HSs.