PTEN expression responds to transcription factor POU and regulates p-AKT levels during diapause initiation in the cotton bollworm, Helicoverpa armigera. (September 2018)

• Record Type: Journal Article
• Authors: Song, Zhe; Yang, Yong-Pan; Xu, Wei-Hua
• Is Part Of: Insect biochemistry and molecular biology. Volume 100(2018)
• Page Start: 48
• Page End: 58
• Publication Date: 2018-09
• Subjects: AKT -- PTEN -- POU -- ROS -- Diapause -- Helicoverpa armigera; Insect biochemistry -- Periodicals; Insects -- Physiology -- Periodicals; Insects -- Molecular aspects -- Periodicals; Biochemistry -- Periodicals; Insectes -- Biochimie -- Périodiques; Insectes -- Composition -- Périodiques; Insectes -- Physiologie -- Périodiques; Insectes -- Aspect moléculaire -- Périodiques; Biochimie -- Périodiques; Biochemistry; Insect biochemistry; Insects -- Molecular aspects; Insects -- Physiology; Periodicals; 572.8157
• DOI: 10.1016/j.ibmb.2018.06.005
• Languages: English
• ISSNs: 0965-1748

Abstract

Abstract: Diapause is a complex physiological response accompanied by many signaling pathways participating in the process. Previous studies have shown that p-AKT levels in brains of diapause-destined pupae are elevated by ROS, and the activated AKT promotes Glut expression for glucose uptake during diapause entry in Helicoverpa armigera . However, the mechanism by which ROS activate AKT is still unclear. Here, we show that PTEN, a PI3K/p-AKT signaling inhibitor, was significantly lower in the brains of diapause-destined pupae and that p-AKT levels were elevated by a lack of PTEN dephosphorylating PIP3. In addition, POU was identified as a transcription factor that binds to the PTEN promoter and regulates its expression. POU expression was enhanced by ecdysone but suppressed by ROS, suggesting that POU/PTEN plays a central role in responding to ROS signaling and regulating p-AKT levels. These results suggest that ecdysone and ROS participate together in the regulation of insect diapause through downregulation of POU/PTEN, which elevates p-AKT levels. Graphical abstract: Image 1 Highlights: PTEN is involved in the regulation of diapause initiation. A buildup of p-AKT through basal PI3K activity due to a lack of PTEN. POU binds to the PTEN promoter and regulates its expression. ROS/ecdysone increase p-AKT levels through downregulation of POU/PTEN.