Improving the lipid recovery from wet oleaginous microorganisms using different pretreatment techniques. (November 2018)

• Record Type: Journal Article
• Authors: Howlader, Md Shamim; Rai, Neeraj; Todd French, William
• Is Part Of: Bioresource technology. Volume 267(2018)
• Page Start: 743
• Page End: 755
• Publication Date: 2018-11
• Subjects: Biofuels -- Oleaginous microbes -- Biomass -- Cell disruption -- Lipid extraction; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2018.07.092
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Mechanical and non-mechanical cell disruption methods are discussed. Lipid recovery of treated wet biomass is compared with conventional method. A novel pretreatment method on wet biomass using pressurized CO2 is presented. New method on cell disruption and lipid extraction from wet biomass is proposed. Abstract: Lipid extraction directly from the wet oleaginous microorganisms for biodiesel production is preferred as it reduces the energy input for traditional processes which require extensive drying of the biomass prior to the extraction. The high water content (≥80% on cell dry weight) in the wet biomass hinders the extraction efficiency due to the mass transfer limitation. This limitation can be overcome by pretreating wet biomass prior to the lipid extraction using pressurized gas that can be used alone or combined with other pretreatments to disrupt the cell wall. In this review, an extensive discussion on different pretreatments and the subsequent lipid extraction using these pretreatments is presented. Furthermore, a detailed account of the cell disruption using pressurized gas (e.g., CO2 ) treatment for microbial cell lysing is also presented. Finally, a new technique on lipid extraction directly from wet biomass using the combination of pressurized CO2 and microwave pretreatment is proposed.