Preparation of a Poly(ethylene glycol)‐Based Cross‐Linked Network from a Click Reaction for Enzyme Immobilization. Issue 20 (27th May 2019) - Oktay, Burcu
Abstract: In this study, a polyethylene glycol (PEG)‐maleimide film was prepared by a thiol‐ene click reaction. The covalent immobilization of amylase was carried out onto the PEG‐maleimide based photo‐curable network via epoxy and C=O groups without any linker. The epoxy and carbonyl groups on the PEG backbone improved the affinity of the enzyme. The immobilization yield of amylase onto the film was 72.46%. The immobilization capacity was also determined as 313 mg/g. The thermal stability of amylase improved via covalent attachment. Immobilized amylase showed maximum activity at 40 °C and pH 6.5. The immobilized amylase continued to retain 66% of its initial activity after 30 days. Abstract : Hetero‐bifunctional PEG was synthesized from hydroxyphenyl maleimide and poly(ethylene glycol) diglycidyl ether. PEG‐maleimide‐epoxy film was prepared by thiol‐ene click chemistry. α‐Amylase was immobilized with one step process onto the PEG‐maleimide film without any coupling agent. Immobilization yield was calculated as 72.46%. Immobilized amylase showed 100% activity at 40 °C and pH 6.5.