Selenized polysaccharides – Biosynthesis and structural analysis. (15th October 2018)

• Record Type: Journal Article
• Authors: Malinowska, Eliza; Klimaszewska, Marzenna; Strączek, Tomasz; Schneider, Krystyna; Kapusta, Czesław; Podsadni, Piotr; Łapienis, Grzegorz; Dawidowski, Maciej; Kleps, Jerzy; Górska, Sandra; Pisklak, Dariusz M.; Turło, Jadwiga
• Is Part Of: Carbohydrate polymers. Volume 198(2018)
• Page Start: 407
• Page End: 417
• Publication Date: 2018-10-15
• Subjects: ATCC American Type Culture Collection -- BRM biological response modifiers -- 13CNMR carbon nuclear magnetic resonance -- COSY correlation spectroscopy -- CR3 (CD11b/CD18) complement receptor 3 -- DB branching degree -- DP degree of polymerization -- 2DNMR two-dimensional nuclear magnetic resonance spectroscopy -- ELSD light-scattering detection/detector -- EXAFS extended X-ray absorption fine structure -- FTIR fourier transform infrared spectroscopy -- GPC gel permeation chromatography -- HPGPC high performance gel permeation liquid chromatography -- HPLC high performance liquid chromatography -- HSQC heteronuclear multiple quantum correlation -- 1HNMR proton nuclear magnetic resonance -- iC3b human neutrophil type 3 complement receptor -- LDC refractive index detector -- Mn number-average molecular weight -- Mp peak molecular weight -- Mw weight-average molecular weight -- NK natural killer cells -- NMR nuclear magnetic resonance spectroscopy -- OPA o-phthaldialdehyde -- PDI polydispersity index -- RALS + LALS laser light scattering detectors -- RPHPLC reverse phase high performance liquid chromatography -- SEC size exclusion chromatography -- Tri-SEC size exclusion chromatography with triple detection -- XAS x-ray absorption spectroscopy -- XANES x-ray absorption near-edge structure; Lentinula edodes -- Selenium -- Se-polysaccharides -- XANES -- EXAFS -- Se-glycosides; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2018.06.057
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: A selenium (Se)-containing polysaccharide-protein fraction was obtained by biosynthesis. Se in the Se-polysaccharide structure was present at the –II oxidation state. Se was organically bound in both the polysaccharide and protein components. Se was glycosidically-linked in either β-1, 3 or α-1, 4-glycosidic bonds or in a pyranoside ring. Molecular modelling predicted structural deformations caused by the Se incorporation. Abstract: The main objective of our research was to analyze the structure of the Se-containing polysaccharides and to examine how the selenium is bound to the polysaccharide molecule. During investigation of the biosynthesis of new immunomodulators, we isolated a selenium (Se)-containing polysaccharide-protein fraction containing proteoglycans of molecular weights of 3.9 × 10 6 Da and 2.6 × 10 5 Da, composed of glucose or mannose, nearly 8% of protein and 190 μg Se/g dry weight. X-ray absorption spectroscopy (XAS) data analysis in the near edge region (XANES) confirmed that selenium in the Se-polysaccharides structure is present at the –II oxidation state and that Se is organically bound. The simulation analysis in the EXAFS (extended X-ray absorption fine structure) region suggested that selenium is most likely bound by a glycosidic-link in a β-1, 3 or α-1, 4-glycosidic bond or substituted for oxygen in a pyranosidic ring. Calculations performed with Gaussian 03 software predicted deformations in the polysaccharide structure caused by the incorporation of the selenium atom including change in bond lengths and torsion angles and, as a result, disappearance of hydrogen bonds in the vicinity of the selenium atoms.