Exopolysaccharide production by an indigenous isolate Pseudomonas stutzeri XP1 and its application potential in enhanced oil recovery. (1st November 2018)

• Record Type: Journal Article
• Authors: Zhao, Feng; Guo, Chao; Cui, Qingfeng; Hao, Qinqin; Xiu, Jianlong; Han, Siqin; Zhang, Ying
• Is Part Of: Carbohydrate polymers. Volume 199(2018)
• Page Start: 375
• Page End: 381
• Publication Date: 2018-11-01
• Subjects: Biopolymer -- Exopolysaccharide -- Rheological property -- Microbial enhanced oil recovery -- Pseudomonas stutzeri; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2018.07.038
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: A rapid and efficient method for screening biopolymer producers was established. A biopolymer producer Pseudomonas stutzeri XP1 was isolated from oil reservoirs. P. stutzeri XP1 can grow and produce 16 g/l exopolysaccharide using corn starch. Exopolysaccharide was identified as dextran with molecular mass of 1.65 × 10 6 Da. The enhanced oil recovery efficiency was 13.56% by in situ metabolism of strain XP1. Abstract: In this study, a rapid and efficient method for screening biopolymer producers was established using 96-well plates. An indigenous biopolymer producer Pseudomonas stutzeri XP1 was isolated from Xinjiang oil reservoirs, China. Strain XP1 can grow and produce 16 g/l biopolymer using corn starch and nitrate. Produced biopolymer increased culture viscosity up to 2384 mPa s. Biopolymer showed rheological properties and pseudo-plastic behavior. The viscosity of 8 g/l biopolymer solution kept higher than 25 mPa s at 20–50 °C and pH values (5–9) and increased to 7600 mPa s with NaCl concentrations increasing to 2%. Gel permeation chromatography data showed that the biopolymer average molecular mass was 1.65 × 10 6 Da. Gas chromatography revealed that the monosaccharide composition in biopolymer was glucose. Core flooding experiments revealed that extra 13.56% of oil was recovered by in situ biopolymer production of strain XP1. Properties of strain XP1 and the biopolymer produced make them promising for enhanced oil recovery.