Characterization and immunoregulatory activity of two polysaccharides from the root of Ilex asprella. (1st October 2018)

• Record Type: Journal Article
• Authors: Meng, Fancheng; Li, Qiu; Qi, Yaning; He, Chengwei; Wang, Chunming; Zhang, Qingwen
• Is Part Of: Carbohydrate polymers. Volume 197(2018)
• Page Start: 9
• Page End: 16
• Publication Date: 2018-10-01
• Subjects: IA Ilex asprella -- IAPS Ilex asprella polysaccharide -- TFA trifluoroacetic acid -- PMP 1 phenyl-3-methyl-5-pyrazolone -- HPSEC-MALLS-RID high performance size exclusion chromatography coupled with multi-angle laser light scattering and refractive index detector -- UV ultraviolet -- IR infrared -- NMR nuclear magnetic resonance; Ilex asprella -- Polysaccharide -- Structure -- Immunoregulatory; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2018.05.066
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: IAPS-2 is one of two polysaccharide fractions from the root of Ilex asprella . IAPS-2 has a branched chain composed of arabinose, rhamnose and galacturonic acid. IAPS-2 increased the secretion of TNF-α, IL-1β, IL-12 at the low dose of LPS. Abstract: The polysaccharides obtained from the root of Ilex asprella, namely IAPS-1 and IAPS-2, with immunoregulatory activity were studied. Two polysaccharides were isolated and purified by Cellulose DEAE-52 and Sephadex columns. The structure of IAPS-1 was elucidated as 1, 6-linked α-d -glucopyranosyl main chain with branch chain substituted at C-2 and/or C-4 position. For IAPS-2, the backbone is composed of 1, 4-linked α-d -glucose, galactose and rhamnose, and branched chains consists of arabinose, rhamnose and galacturonic acid, as confirmed by partial acid hydrolysis and NMR study. Further, the immunoregulatory activity of IAPS-1 and IAPS-2 was tested with the murine macrophages. Particularly, IAPS-2 polysaccharide can more effectively enhance the secretion of major inflammatory cytokines in macrophages, such as TNF-α, IL-1β, IL-12, compared with IAPS-1.