Rhizopus nigricans polysaccharide activated macrophages and suppressed tumor growth in CT26 tumor-bearing mice. (15th October 2018)

• Record Type: Journal Article
• Authors: Wei, Zhihong; Chen, Guochuang; Zhang, Pengying; Zhu, Lei; Zhang, Linan; Chen, Kaoshan
• Is Part Of: Carbohydrate polymers. Volume 198(2018)
• Page Start: 302
• Page End: 312
• Publication Date: 2018-10-15
• Subjects: MAPKs mitogen-activated protein kinases -- NF-κB nuclear factor-κB -- NO nitric oxide -- HPSEC-MALLS high performance size-exclusion chromatography with multiangle laser light scattering -- PMB polymyxin B -- 5-FU 5-fluorouracil; Polysaccharides -- Rhizopus nigricans -- Macrophage -- Antitumor -- CT26 colon cancer; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2018.06.076
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A homogeneous polysaccharide (RPS-1) was extracted from Rhizopus nigricans . RPS-1 was non-starch α-1, 6 branched α-1, 4-glucan and its Mw was 1.617 × 10 7 g/mol. MAPKs and NF-κB pathways were involved in macrophages activation induced by RPS-1. Intragastric administration of RPS-1 inhibited tumor growth of CT26 tumor-bearing mice. Abstract: In this study, a homogeneous polysaccharide (RPS-1) was extracted from liquid-cultured mycelia of Rhizopus nigricans . The weight-average molecular weight of RPS-1 was 1.617 × 10 7 g/mol and structural characterization indicated that RPS-1 was a non-starch glucan which consisted of a backbone structure of (1→4)-linked α-d -glucopyranosyl residues substituted at the O-6 position with α-d -glucopyranosyl branches. RPS-1 stimulated the production of nitric oxide and tumor necrosis factor-α by triggering phosphorylation of mitogen-activated protein kinases and nuclear translocation of nuclear factor kappa B p65 in RAW 264.7 macrophage cells. Moreover, intragastric administration of RPS-1 improved the immune function of CT26 tumor-bearing mice and significantly inhibited the growth of transplanted tumor. In combination with 5-FU, RPS-1 enhanced antitumor activity of 5-FU and alleviated its toxicity on immune system. These findings suggested that RPS-1 has the potential for the development of functional foods and dietary supplements.