Structural characterization and antioxidant activity of water-soluble polysaccharides from the Tunisian brown seaweed Cystoseira compressa. (15th October 2018)

• Record Type: Journal Article
• Authors: Hentati, Faiez; Delattre, Cédric; Ursu, Alina V.; Desbrières, Jacques; Le Cerf, Didier; Gardarin, Christine; Abdelkafi, Slim; Michaud, Philippe; Pierre, Guillaume
• Is Part Of: Carbohydrate polymers. Volume 198(2018)
• Page Start: 589
• Page End: 600
• Publication Date: 2018-10-15
• Subjects: Brown seaweed -- Cystoseira -- Sulfated polysaccharide -- Alginate -- Fucoidan -- Antioxidant activity; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2018.06.098
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Two water-soluble polysaccharides were extracted from the Tunisian brown alga C. compressa . The sulfated fucoidan was a galactofucan with a backbone of α -(1, 3) and α -(1, 4)-l -Fuc p, highly substituted. The sodium alginate showed a M/G ratio closed to 0.77 and the ability to form solid and rigid gel. The polysaccharides displayed antioxidant activities with IC50 values under 560 μg/mL. Abstract: A fucoidan (CCF) and a sodium alginate (CCSA) were extracted and purified from the Tunisian brown seaweed Cystoseira compressa . CCF was a highly sulfated heterogalactofucan composed of α -(1→3), α -(1→4)-linked l -Fuc p as main backbone which could be highly branched (31.84%) at O -3 and O -4 positions of α -(1→4)-l -Fuc p and α -(1→3)-l -Fuc p by terminal monosaccharides and side chains such as terminal α -l -Fuc p, terminal β -d -Gal p, β -d -Gal p -(1→3)- α -l -Fuc p and β -d -Gal p -(1→4)- α -l -Fuc p . The ratio of α -(1→3)/ α -(1→4) linkages was estimated at 3.86:1. CCSA was characterized by HPAEC-PAD, GC/MS-EI, ATR-FTIR, and 1 H-NMR. The M/G ratio was M/G = 0.77, indicating that CCSA respectively contained 44% and 56% of mannuronic and guluronic acids. The values of FGG, FMM, FGM (or FMG ) blocks as well as the parameter η were estimated. The two polysaccharides exhibited effective antioxidant activities by ferrous ion chelation, ferric ion reduction and DPPH radical-scavenging, outlining their potentials as natural additives.