Spectroscopic investigation of a hyperbranched cationic amylopectin derivative as a multi-guest molecular host for targeted delivery of a photosensitizer to pancreatic cancer cells. (1st October 2018)

• Record Type: Journal Article
• Authors: Tan, Jiayun; Wang, Xiaojuan; Yu, Zhong; Luo, Jiahao; Lan, Biyun; Li, Na; Xin, Yachuan; Zeng, Chunlian; Yan, Li; Zhang, Li-Ming; Chen, Xiaohong; Guan, Shanyue; Li, Wei; Yang, Liqun
• Is Part Of: Carbohydrate polymers. Volume 197(2018)
• Page Start: 38
• Page End: 46
• Publication Date: 2018-10-01
• Subjects: Amylopectin -- Photosensitizer -- Complexation mechanism -- NMR spectroscopy -- Fluorescence spectroscopy; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2018.05.051
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A hyperbranched amylopectin derivative as a host for photosensitizer and folic acid. The complex showed high phototoxicity against human pancreatic cancer cells. A method to study hyperbranched polymers as multi-functional drug delivery carriers. Abstract: To increase pancreatic tumor-targeted phototoxicity of photosensitizers, a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp) was invevstigated as a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells. We selected protoporphyrin IX (PpIX) and folic acid as a photosensitizer and a tumor-targeting factor, respectively. The complexation mechanism of DMAPA-Amp with PpIX and folic acid was characterized using NMR spectroscopy including 1 H NMR, two-dimensional diffusion ordered spectroscopy (2D DOSY) NMR, fluorescence and UV–vis spectroscopy. The results indicated that the DMAPA-Amp derivative could serve as a host for the encapsulation of two guests, PpIX and folic acid, through intermolecular interactions. The complex showed high phototoxicity against Panc-1 cells, and its folic-acid-mediated cancer-cell-targeting property was confirmed by laser confocal microscopy and flow cytometry analysis. We provide a method to study hyperbranched cationic polymer-based complexes containing multiple guests, which could facilitate the design of multi-functional complexes in the drug delivery field.