HPLC analysis, optimization of extraction and purification conditions, biological evaluation of total protoflavones from Macrothelypteris viridifrons. Issue 21 (2nd November 2019)

• Record Type: Journal Article
• Authors: Wei, An-Hua; Zhou, Dao-Nian; Gu, Zhi-Chun; Liu, Dong
• Is Part Of: Natural product research. Volume 33:Issue 21(2019)
• Page Start: 3167
• Page End: 3170
• Publication Date: 2019-11-02
• Subjects: Macrothelypteris viridifrons -- HPLC -- protoflavone -- protoapigenone -- anti-tumor; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2018.1522312
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: The present study aims to evaluate phytochemical and pharmacological potential of total protoflavones from Macrothelypteris viridifrons . In the phytochemical study, an HPLC analysis method was established, and the optimal extraction and purification conditions were analyzed. The extractive condition was optimized as follows: the backflow extraction with 20 folds of 70% ethanol at 80◦C for 1 h twice. Moreover, by combining the alkali-extraction and acid-precipitation method with the macroporous resin purification technology, the final purity rate of total protoflavones was no less than 54.85%. In the pharmacological study, the total protoflavones from M. viridifrons showed a significant tumor-inhibitory effect in the H22 hepatoma cells transplantation model with a higher inhibitory rate of 55.76% in high dosage (100mg/kg) treatment group compared with the positive control group (20 mg/kg cyclophosphamide). Taken of all, these results support that protoflavones are the material basis of M. viridifrons as an anticancer folk medicine. Graphical Abstract: