Significant Association of HLA-B Alleles and Genotypes in Thai Children with Autism Spectrum Disorders: A Case-Control Study. (24th December 2015)

• Record Type: Journal Article
• Authors: Puangpetch, Apichaya; Suwannarat, Pongwut; Chamnanphol, Montri; Koomdee, Napatrupron; Ngamsamut, Nattawat; Limsila, Penkhae; Sukasem, Chonlaphat
• Other Names: Chace Donald H. Academic Editor.
• Is Part Of: Disease markers. Volume 2015(2015)
• Publication Date: 2015-12-24
• Subjects: Diagnosis -- Periodicals; Biochemical markers -- Periodicals; Pathology -- Periodicals; 616
• DOI: 10.1155/2015/724935
• Languages: English
• ISSNs: 0278-0240

Abstract

Abstract : Autism is a severe neurodevelopmental disorder. Many susceptible causative genes have been identified. Most of the previous reports showed the relationship between the Human Leukocyte Antigen (HLA) gene and etiology of autism. In order to identify HLA-B alleles associated with autism in Thai population, we compared the frequency of HLA-B allele in 364 autistic subjects with 952 normal subjects by using a two-stage sequence-specific oligonucleotide probe system (PCR-SSOP) method based on flow-cytometry technology. HLA- B ⁎ 13:02 (P = 0.019, OR = 2.229), HLA- B ⁎ 38:02 (P = 0.049, OR = 1.628), HLA- B ⁎ 44:03 (P = 0.016, OR = 1.645), and HLA- B ⁎ 56:01 (P = 1.78 × 10 −4, OR = 4.927) alleles were significantly increased in autistic subjects compared with normal subjects. Moreover, we found that the HLA- B ⁎ 18:02 (P = 0.016, OR = 0.375) and HLA- B ⁎ 46:12 (P = 0.008, OR = 0.147) alleles were negatively associated with autism when compared to normal controls. Both alleles might have a protective role in disease development. In addition, four HLA-B genotypes of autistic patients had statistically significant relationship with control groups, consisting of HLA- B ⁎ 3905 / ⁎ 5801 (P = 0.032, OR = 24.697), HLA- B ⁎ 2704 / ⁎ 5801 (P = 0.022, OR = 6.872), HLA- B ⁎ 3501 / ⁎ 4403 (P = 0.021, OR = 30.269), and HLA- B ⁎ 1801 / ⁎ 4402 (P = 0.017, OR = 13.757). This is the first report on HLA-B associated with Thai autism and may serve as a marker for genetic susceptibility to autism in Thai population.