In vitro analysis of dextran from Leuconostoc mesenteroides NRRL B-1426 for functional food application. Issue 2 (October 2015)

• Record Type: Journal Article
• Authors: Kothari, Damini; Tingirikari, Jagan Mohan Rao; Goyal, Arun
• Is Part Of: Bioactive carbohydrates and dietary fibre. Volume 6:Issue 2(2015)
• Page Start: 55
• Page End: 61
• Publication Date: 2015-10
• Subjects: Dextran -- Biocompatible -- Leuconostoc mesenteroides -- Prebiotic; Functional foods -- Periodicals; Fiber in human nutrition -- Periodicals; Carbohydrates -- Periodicals; Glycoproteins -- Periodicals; Functional Food -- Periodicals; Dietary Fiber -- Periodicals; Carbohydrates -- Periodicals; Carbohydrates; Fiber in human nutrition; Functional foods; Glycoproteins; Periodicals; 613.28
• DOI: 10.1016/j.bcdf.2015.08.001
• Languages: English
• ISSNs: 2212-6198

Abstract

Abstract: The present study describes physico-chemical properties, prebiotic potential and biocompatibility of dextran produced from Leuconostoc mesenteroides NRRL B-1426. Dextran displayed porous surface, pseudoplastic non-Newtonian behavior, 32% solubility, 290% water holding capacity and a degradation temperature of 290 °C. The dextran exhibited significantly higher non digestibility as compared with the standard prebiotic, inulin in simulated human gastric juice and α-amylase. The dextran significantly stimulated the growth of probiotics, bifidobacteria and lactobacilli and was comparable to that by inulin. The viability of human embryonic kidney (HEK 293), human cervical adenocarcinoma (INT-407) and human colon carcinoma (HT-29) cell lines by dextran treatment (50–1000 µg/ml) remained unaffected displaying the biocompatibility of the dextran. Therefore, the dextran from L. mesenteroides NRRL B-1426 can act as potential prebiotic, gelling agent and be used as food supplement for health benefits. Graphical abstract: Highlights: High thermal stability of dextran with a degradation temperature of ~290 °C. Dextran exhibited in vitro prebiotic activity. In vitro cytotoxicity assay reveals biocompatibility nature of dextran.