Development and evaluation of a Luminex xTAG assay for sulfonamide resistance genes in Escherichia coli and Salmonella isolates. (February 2020)

• Record Type: Journal Article
• Authors: Xu, Fengjiao; Min, Fangui; Wang, Jing; Luo, Yinzhu; Huang, Shuwu; Chen, Meiling; Wu, Ruike; Zhang, Yu
• Is Part Of: Molecular and cellular probes. Volume 49(2020)
• Publication Date: 2020-02
• Subjects: Luminex xTAG assay -- sul genes -- Sulfonamide resistance -- Escherichia coli -- Salmonella isolates; Molecular probes -- Diagnostic use -- Periodicals; Pathology, Cellular -- Technique -- Periodicals; Cell Biology -- Periodicals; Molecular Biology -- Periodicals; Sondes moléculaires -- Utilisation diagnostique -- Périodiques; Cytopathologie -- Technique -- Périodiques; 572
• DOI: 10.1016/j.mcp.2019.101476
• Languages: English
• ISSNs: 0890-8508

Abstract

Abstract: Clinically occurring sulfonamide resistance in gram-negative bacteria is codified by several sul genes, mostly associated with the mobilized genetic elements named integrons, and integrons are frequently found in plasmids. There are four sul genes ( sul1, sul2, sul3 and sul4 ) that encode resistance to sulfonamides. The aim of the present study was to develop a bead-based xTAG assay for the simultaneous detection of all four sul genes and related Class 1 integrons ( int1 ) in Escherichia coli and Salmonella isolates. The limits of detection ranged from 10 to 1000 copies/μL of input purified plasmid DNA. Forty-one bacterial isolates from clinical samples were examined using the newly developed xTAG assay and also by conventional PCR to determine the relative performance of each. The results obtained by xTAG assay showed higher detection rates and accuracy for sul genes than conventional PCR. It indicated that the xTAG-multiplex PCR is a convenient method for rapid identification of sul genes. Highlights: A Luminex xTAG assay was developed for rapid identification of sul genes. Detection limits of four sul genes and related int1 were from 10 to 1000 copies/μL. Compared to conventional PCR, it showed higher detection rates and accuracy.