Armitage determines Piwi−piRISC processing from precursor formation and quality control to inter‐organelle translocation. (12th December 2019)

• Record Type: Journal Article
• Authors: Yamashiro, Haruna; Negishi, Mayu; Kinoshita, Tatsuki; Ishizu, Hirotsugu; Ohtani, Hitoshi; Siomi, Mikiko C
• Is Part Of: EMBO reports. Volume 21:Number 2(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-12-12
• Subjects: Armitage -- Gasz -- mitochondrion -- piRNA -- Yb body; Molecular biology -- Periodicals; Molecular Biology -- Periodicals; Molecular biology; Periodicals; 572.8
• DOI: 10.15252/embr.201948769
• Languages: English
• ISSNs: 1469-221X

Abstract

Abstract: Piwi and piRNA form the piRNA‐induced silencing complex (piRISC) to repress transposons. In the current model, Armitage (Armi) brings the Piwi–piRISC precursor (pre‐piRISC) to mitochondria, where Zucchini‐dependent piRISC maturation occurs. Here, we show that Armi is necessary for Piwi–pre‐piRISC formation at Yb bodies and that Armi triggers the exit of Piwi–pre‐piRISC from Yb bodies and the translocation to mitochondria. Piwi–pre‐piRISC resist leaving Yb bodies until Armi binds Piwi–pre‐piRISC through the piRNA precursors. The lack of the Armi N‐terminus also blocks the Piwi–pre‐piRISC exit from Yb bodies. Thus, Armi determines Piwi–piRISC processing, in a multilayered manner, from precursor formation and quality control to inter‐organelle translocation for maturation. Synopsis: Armitage determines Piwi–piRISC processing, in a multilayered manner, from precursor formation and quality control to inter‐organelle translocation for Zucchini‐dependent piRISC maturation on mitochondria. Armi is necessary for Piwi–pre‐piRISC formation at Yb bodies, perinuclear non‐membranous organelles. Armi senses Piwi–pre‐piRISC assembly and triggers the exit of the complex from Yb bodies. The RNA‐binding activity and the N‐terminus of Armi are required for the translocation of Piwi–pre‐piRISC from Yb body to mitochondria. Abstract : Armitage determines Piwi–piRISC processing, in a multilayered manner, from precursor formation and quality control to inter‐organelle translocation for Zucchini‐dependent piRISC maturation on mitochondria.