Chromobacterium violaceum delivers violacein, a hydrophobic antibiotic, to other microbes in membrane vesicles. (2nd January 2020)

• Record Type: Journal Article
• Authors: Choi, Seong Yeol; Lim, Sungbin; Cho, Gayoung; Kwon, Jisoo; Mun, Wonsik; Im, Hansol; Mitchell, Robert J.
• Is Part Of: Environmental microbiology. Volume 22:Number 2(2020)
• Page Start: 705
• Page End: 713
• Publication Date: 2020-01-02
• Subjects: Microbial ecology -- Periodicals; Environmental Microbiology -- Periodicals; 579.17
• DOI: 10.1111/1462-2920.14888
• Languages: English
• ISSNs: 1462-2912

Abstract

Summary: This study describes Chromobacterium violaceum 's use of extracellular membrane vesicles (MVs) to both solubilize and transport violacein to other microorganisms. Violacein is a hydrophobic bisindole with known antibiotic activities against other microorganisms. Characterization of the MVs found they carried more violacein than protein (1.37 ± 0.19‐fold), suggesting they may act as a reservoir for this compound. However, MVs are not produced in response to violacein – a Δ vioA isogenic mutant, which is incapable of making violacein, actually produced significantly more MVs (3.2‐fold) than the wild‐type strain. Although violacein is insoluble in water (Log Poctanol:water = 3.34), 79.5% remained in the aqueous phase when it was present within the C. violaceum MVs, an increase in solubility of 1740‐fold. Moreover, tests with a strain of Staphylococcus aureus showed MV‐associated violacein is bactericidal, with 3.1 mg/l killing 90% of S. aureus in 6 h. Tests with the Δ vioA MVs found no loss in the S. aureus viability, even when its MVs were added at much higher concentrations, demonstrating violacein is the active component within the wild‐type MVs. In conclusion, our study clearly demonstrates C. violaceum produces MVs and uses them as vehicles to solubilize violacein and transport this hydrophobic antibiotic to other microbes.