Enhanced function of chondrocytes in a chitosan‐based hydrogel to regenerate cartilage tissues by accelerating degradability of the hydrogel via a hydrolysable crosslinker. Issue 19 (31st December 2019)

• Record Type: Journal Article
• Authors: Ishikawa, Shohei; Iijima, Kazutoshi; Matsukuma, Daisuke; Iijima, Michihiro; Osawa, Shigehito; Otsuka, Hidenori
• Is Part Of: Journal of applied polymer science. Volume 137:Issue 19(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-12-31
• Subjects: biodegradable -- biomaterials -- biomedical applications -- gels; Polymers -- Periodicals; Polymerization -- Periodicals; 668.9
• DOI: 10.1002/app.48893
• Languages: English
• ISSNs: 0021-8995

Abstract

ABSTRACT: Chitosan‐based hydrogels as scaffolds for culturing chondrocytes were prepared using linkers with and without hydrolysable poly(dl ‐lactide) (PLA) segments. The evaluation of the cultured chondrocytes in them indicated that the accelerated degradation of the hydrogel via hydrolysis of the PLA slightly promoted production of the sulfated glycosaminoglycan and drastically improved that of collagen from the encapsulated chondrocytes, which are the chondrospecific extracellular matrix components. Furthermore, the accelerated degradability significantly upregulated the gene expression for Collagen II production and downregulated that for Collagen I production of the encapsulated chondrocytes. Because major component of the articular cartilage tissue is Collagen II‐rich hyaline cartilage, these results suggest the degradation of the scaffolds is an important parameter in cartilage tissue regeneration and the accelerated degradability may have benefits on promotion of cartilage tissue regeneration especially from the viewpoint of hyaline cartilage‐like collagen production. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 137, 48893. Abstract : Chitsan‐based hydrogel with accelerated degradability, which is based on the gradual hydrolysis of cross‐linkers, was applied to scaffold for chondrocytes, enhancing generation of cartilage‐forming protein including collagen and proteoglycan.