Metformin decreases testicular damages following ischaemia/reperfusion injury in rats. (9th December 2019)

• Record Type: Journal Article
• Authors: Sarıbal, Devrim; Erdem, Erkan; Güngör‐Ordueri, Nazlı Ece; Usta, Akın; Karakuş, Cemil; Karacan, Meriç
• Is Part Of: Andrologia. Volume 52:Number 2(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-12-09
• Subjects: apoptosis -- cleaved caspase‐3 -- metformin -- testicular ischaemia/reperfusion -- torsion/detorsion; Andrology -- Periodicals; Infertility, Male -- Periodicals; 616.69
• DOI: 10.1111/and.13481
• Languages: English
• ISSNs: 0303-4569

Abstract

Abstract: The effects of metformin on a testicular torsion injury in adolescent rat testis after I/R were evaluated in the present study. Forty adolescent rats were divided into five groups with eight rats per group: a control group; a sham‐operated group; an ischaemia group, where torsion was applied for 4 hr and testis was examined immediately after detorsion; an I/R group, where torsion was applied for 4 hr and the testis was examined 4 hr after detorsion; and an I/R + M group, where the metformin (300 mg/kg) administration was added to the identical procedures used for the I/R group. Spermatogenesis, basal membrane integrity and cleaved caspase‐3 expression were assessed. The I/R + M group had a significantly higher Johnsen score than the I/R group (7.9 ± 0.1 vs. 7.5 ± 0.2; p < .001; F ‐value = 14.2). Failure of basal membrane integrity was highest in the ischaemia group (45 ± 5) compared to the other groups (control group, 20 ± 5; sham‐operated group, 16.6 ± 2.8), but not different between the I/R + M (31.6 ± 12.5) and the I/R groups (25 ± 3.5). Cleaved caspase‐3 expression was highest in the ischaemia group (73.5 ± 0.7), and significantly lower in the I/R + M group (33.4 ± 0.9) than the I/R group (58.5 ± 0.2; p < .05; F ‐value = 7.6). Metformin decreases testicular damage by exerting protection against the harmful effects of I/R on spermatogenesis and alleviating apoptosis in adolescent rat testis.