Evolutionary changes of an intestinal Lactobacillus reuteri during probiotic manufacture. Issue 2 (19th November 2019)

• Record Type: Journal Article
• Authors: López, Lázaro; Calderón, Diana; Cardenas, Paúl; Prado, María B.; Valle, Carlos; Trueba, Gabriel
• Is Part Of: MicrobiologyOpen. Volume 9:Issue 2(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-11-19
• Subjects: antagonistic pleiotropy -- experimental evolution -- Lactobacillus reuteri -- probiotics; Microbiology -- Periodicals; 579
• DOI: 10.1002/mbo3.972
• Languages: English
• ISSNs: 2045-8827

Abstract

Abstract: Probiotic bacteria are frequently used to treat intestinal diseases or to improve health; however, little is known about the evolutionary changes of these bacteria during probiotic manufacture and the bacterial ability to colonize the intestine. It has been observed that when bacteria adapt to a new environment, they lose some traits required to thrive in the original niche. In this study, a strain of Lactobacillus reuteri was isolated from mouse duodenum and subjected to 150 serial passes in milk to simulate the industrial propagation of probiotic bacteria. The strains adapted to milk outperformed their ancestor when grown in milk; we also showed evidence of reduced intestinal colonization of milk‐adapted strains. Whole‐genome sequencing showed that bacterial adaptation to milk selects mutants with altered metabolic functions. Abstract : We simulated the evolution of a probiotic bacteria. We isolated an intestinal Lactobacillus reuteri (from mouse) and subjected it to 150 passes in milk. The strain adapted to milk gained ability to grow in this substrate, but it seemed to have lost the ability to colonize intestines.