Evaluation of swine enteroids as in vitro models for Lawsonia intracellularis infection1, 2. (16th January 2020)

• Record Type: Journal Article
• Authors: Resende, Talita Pilar; Medida, Ramya Lekha; Vannucci, Fabio A; Saqui-Salces, Milena; Gebhart, Connie
• Is Part Of: Journal of animal science. Volume 98:Number 2(2020)
• Publication Date: 2020-01-16
• Subjects: in vitro model -- intracellular bacteria -- Lawsonia intracellularis -- organoids -- pathogenesis -- proliferative enteropathy; Livestock -- Periodicals; Livestock; Electronic journals; Periodicals; 636.005
• DOI: 10.1093/jas/skaa011
• Languages: English
• ISSNs: 0021-8812

Abstract

Abstract: The enteric pathogen Lawsonia intracellularis is one of the main causes of diarrhea and compromised weight gain in pigs worldwide. Traditional cell-line cultures have been used to study L. intracellularis pathogenesis. However, these systems fail to reproduce the epithelial changes observed in the intestines of L. intracellularis -infected pigs, specifically, the changes in intestinal cell constitution and gene expression. A more physiologically accurate and state-of-the-art model is provided by swine enteroids derived from stem cell-containing crypts from healthy pigs. The objective of this study was to verify the feasibility of two-dimensional swine enteroids as in vitro models for L. intracellularis infection. We established both three- and two-dimensional swine enteroid cultures derived from intestinal crypts. The two-dimensional swine enteroids were infected by L. intracellularis in four independent experiments. Enteroid-infected samples were collected 3 and 7 d postinfection for analysis using real-time quantitative PCR and L. intracellularis immunohistochemistry. In this study, we show that L. intracellularis is capable of infecting and replicating intracellularly in two-dimensional swine enteroids derived from ileum.