Mechanobiological modulation of in situ and in vivo osteocyte calcium oscillation by acoustic radiation force. Issue 1 (23rd October 2019)

• Record Type: Journal Article
• Authors: Hu, Minyi; Lee, Wonsae; Jiao, Jian; Li, Xiaofei; Gibbons, Daniel E.; Hassan, Chaudhry Raza; Tian, Guo‐Wei; Qin, Yi‐Xian
• Editors: Sun, Hui B.
• Is Part Of: Annals of the New York Academy of Sciences. Volume 1460:Issue 1(2020)
• Page Start: 68
• Page End: 76
• Publication Date: 2019-10-23
• Subjects: low‐intensity pulsed ultrasound (LIPUS) -- acoustic radiation force -- cellular mechanics -- Wnt signaling -- Ca2+ release -- fluid flow; Medical sciences -- Periodicals; Medicine -- Periodicals; Science -- Periodicals; 610
• DOI: 10.1111/nyas.14262
• Languages: English
• ISSNs: 0077-8923

Abstract

Abstract: The biological effect of ultrasound on bone regeneration has been well documented, yet the underlying mechanotransduction mechanism is largely unknown. In relation to the mechanobiological modulation of the cytoskeleton and Ca 2+ influx by short‐term focused acoustic radiation force (FARF), the current study aimed to visualize and quantify Ca 2+ oscillations in real‐time of in situ and in vivo osteocytes in response to focused low‐intensity pulsed ultrasound (FLIPUS). For in situ studies, fresh mice calvaria were subjected to FLIPUS stimulation at 0.05, 0.2, 0.3, and 0.7 W. For the in vivo study, 3‐month‐old C57BL/6J Ai38/Dmp1‐Cre mice were subjected to FLIPUS at 0.15, 1, and 1.5 W. As observed via real‐time confocal imaging, in situ FLIPUS led to more than 80% of cells exhibiting Ca 2+ oscillations at 0.3–0.7 W and led to a higher number of Ca 2+ spikes with larger values at >0.3 W. In vivo FLIPUS at 1–1.5 W led to more than 90% of cells exhibiting Ca 2+ oscillations. Higher FLIPUS energies led to larger Ca 2+ spike magnitudes. In conclusion, this study provided a pilot study of both in situ and in vivo osteocytic Ca 2+ oscillations under noninvasive FARF, which aids further exploration of the mechanosensing mechanism of the controlled bone cell motility response to the stimulus. Abstract : The mechanism underlying the effect of ultrasound on bone regeneration is largely unknown. This paper provides a pilot study of the modulation by noninvasive focused acoustic radiation force (FARF) of Ca 2+ oscillations in both in situ and in vivo osteocytes, which will aid further exploration of the mechanosensing mechanism of the controlled bone cell motility response to ultrasound.