Hydrogen produced in rat colon improves in vivo reduction–oxidation balance due to induced regeneration of α-tocopherol. Issue 5 (3rd December 2019)

• Record Type: Journal Article
• Authors: Ishida, Yosuke; Hino, Shingo; Morita, Tatsuya; Ikeda, Saiko; Nishimura, Naomichi
• Is Part Of: British journal of nutrition. Volume 123:Issue 5(2020)
• Page Start: 537
• Page End: 544
• Publication Date: 2019-12-03
• Subjects: Hydrogen molecules, -- α-Tocopherol, -- Adipose tissue, -- Colon, -- Rats; Nutrition -- Periodicals; 572.4
• DOI: 10.1017/S0007114519003118
• Languages: English
• ISSNs: 0007-1145

Abstract

Abstract: We investigated whether non-digestible saccharide fermentation-derived hydrogen molecules (H2 ) in rat colon could improve the in vivo reduction–oxidation (redox) balance via regeneration of α -tocopherol, by assessing their effect on hydroxyl radicals, the α -tocopherol concentration and the redox balance. In Expt 1, a Fenton reaction with phenylalanine (0 or 1·37 mmol/l of H2 ) was conducted. In Expt 2, rats received intraperitoneally maize oil containing phorone (400 mg/kg) 7 d after drinking ad libitum water containing 0 or 4 % fructo-oligosaccharides (FOS) (groups CP and FP, respectively). In Expt 3, rats unable to synthesise ascorbic acid drank ad libitum for 14 d water with 240 mg ascorbic acid/l (group AC), 20 mg of ascorbic acid/l (group DC) or 20 mg of ascorbic acid/l and 4 % FOS (group DCF). In the Fenton reaction, H2 reduced tyrosine produced from phenylalanine to 72 % when platinum was added and to 92 % when platinum was excluded. In Expt 2, liver glutathione was depleted by administration of phorone to rats. However, compared with CP, no change in the m -tyrosine concentration in the liver of FP was detected. In Expt 3, net H2 excretion was higher in DCF than in the other rats after 3 d of the experiment. Furthermore, the concentrations of H2 and α -tocopherol and the redox glutathione ratio in perirenal adipose tissue of rats were significantly higher in DCF than in DC. To summarise, in rat colon, fermentation-derived H2 further shifted the redox balance towards a more reducing status in perirenal adipose tissue through increased regeneration of α -tocopherol.