Single molecule characterization of individual extracellular vesicles from pancreatic cancer. Issue 1 (1st December 2019)

• Record Type: Journal Article
• Authors: Lennon, Kathleen M.; Wakefield, Devin L.; Maddox, Adam L.; Brehove, Matthew S.; Willner, Ari N.; Garcia-Mansfield, Krystine; Meechoovet, Bessie; Reiman, Rebecca; Hutchins, Elizabeth; Miller, Marcia M.; Goel, Ajay; Pirrotte, Patrick; Van Keuren-Jensen, Kendall; Jovanovic-Talisman, Tijana
• Is Part Of: Journal of extracellular vesicles. Volume 8:Issue 1(2019)
• Publication Date: 2019-12-01
• Subjects: Quantitative single molecule localization microscopy -- extracellular vesicles -- pancreatic cancer -- translational medicine -- imaging -- diagnostics; Cells -- Mechanical properties -- Periodicals; Transport Vesicles; Cells -- Mechanical properties; Periodicals; Fulltext; Internet Resources; Periodicals; Electronic journals; Periodicals; 571.63
• DOI: 10.1080/20013078.2019.1685634
• Languages: English
• ISSNs: 2001-3078

Abstract

ABSTRACT: Biofluid-accessible extracellular vesicles (EVs) may represent a new means to improve the sensitivity and specificity of detecting disease. However, current methods to isolate EVs encounter challenges when they are used to select specific populations. Moreover, it has been difficult to comprehensively characterize heterogeneous EV populations at the single vesicle level. Here, we robustly assessed heterogeneous EV populations from cultured cell lines via nanoparticle tracking analysis, proteomics, transcriptomics, transmission electron microscopy, and quantitative single molecule localization microscopy (qSMLM). Using qSMLM, we quantified the size and biomarker content of individual EVs. We applied qSMLM to patient plasma samples and identified a pancreatic cancer-enriched EV population. Our goal is to advance single molecule characterization of EVs for early disease detection. Abbreviations : EV: Extracellular Vesicle; qSMLM: quantitative Single Molecule Localization Microscopy; PDAC: Pancreatic Ductal Adenocarcinoma; EGFR: epidermal growth factor receptor 1; CA19-9: carbohydrate antigen 19-9; SEC: size exclusion chromatography; WGA: wheat germ agglutinin; AF647: Alexa Fluor 647; Ab: antibody; HPDEC: Healthy Pancreatic Ductal Epithelial Cell; TEM: Transmission Electron Microscopy.