Development of an LC‐MS/MS method for quantification of kadsurenone in rat plasma and its application to a pharmacokinetic study. (11th July 2013)

• Record Type: Journal Article
• Authors: Zhang, Nan; Li, Rui; Yu, Haiyao; Shi, Dan; Dong, Ning; Zhang, Sixi; Wang, Helei
• Is Part Of: Biomedical chromatography. Volume 27:Number 12(2013:Dec.)
• Page Start: 1754
• Page End: 1758
• Publication Date: 2013-07-11
• Subjects: kadsurenone -- Piper kadsura -- Alzheimer's disease -- pharmacokinetic -- rat plasma; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.2989
• Languages: English
• ISSNs: 0269-3879

Abstract

ABSTRACT: A sensitive and rapid LC‐MS/MS method was developed and validated for the determination of kadsurenone in rat plasma using lysionotin as the internal standard (IS). The analytes were extracted from rat plasma with acetonitrile and separated on a SB‐C18 column (50 × 2.1 mm, i.d.; 1.8 µm) at 30 °C. Elution was achieved with a mobile phase consisting of methanol–water–formic acid (65:35:0.1, v/v/v) at a flow rate of 0.30 mL/min. Detection and quantification for analytes were performed by mass spectrometry in the multiple reaction monitoring mode with positive electrospray ionization m/z at 357.1 → 178.1 for kadsurenone, and m/z 345.1 → 315.1 for IS. Calibration curves were linear over a concentration range of 4.88–1464 ng/mL with a lower limit of quantification of 4.88 ng/mL. The intra‐ and inter‐day accuracies and precisions were <8.9%. The LC‐MS/MS assay was successfully applied for oral pharmacokinetic evaluation of kadsurenone using the rat as an animal model. Copyright © 2013 John Wiley & Sons, Ltd.