Assessing the progression of gastric cancer via profiling of histamine, histidine, and bile acids in gastric juice using LC-MS/MS. Issue 197 (March 2020)

• Record Type: Journal Article
• Authors: Lee, Wonwoong; Um, Jinhee; Hwang, Boram; Lee, Yong Chan; Chung, Bong Chul; Hong, Jongki
• Is Part Of: Journal of steroid biochemistry and molecular biology. Issue 197(2020)
• Publication Date: 2020-03
• Subjects: Bile acids -- Histidine decarboxylase -- LC-MS/MS -- Gut microbiome -- Gastric juice -- Gastric cancer; Steroid hormones -- Periodicals; Biochemistry -- Periodicals; Hormones -- Periodicals; Molecular Biology -- Periodicals; Hormones stéroïdes -- Périodiques; Steroid hormones; Periodicals; 572.579
• DOI: 10.1016/j.jsbmb.2019.105539
• Languages: English
• ISSNs: 0960-0760

Abstract

Highlights: A new LC-MS/MS method was established for diagnosis of the progress of gastric diseases. Diagnosis of progress of gastric diseases was assessed by profiling of histamine, histidine, and bile acids in gastric juice. Altered metabolism of bile acids in IM and gastric cancer was observed, compared to CSG. The ratio of DCA/CA could be used to diagnose the progression from CSG to IM or gastric cancer. Abstract: Bile acid (BA) imbalance may be directly associated with gastric cancer and indirectly influence stomach carcinogenesis via overexpression of histidine decarboxylase (HDC), which converts histidine (His) into histamine (HIST). Moreover, the progression of gastric cancer, could change the gut microbiome, including bacteria spp. that produce secondary BAs. Gastric juice has various metabolites that could indicate gastric cancer-related stomach conditions. Therefore, profiling of HIST, His, and BAs in gastric juice is crucial for understanding the etiological mechanisms of gastric cancer. We used a profiling method to simultaneously determine targeted metabolites in gastric juice using liquid chromatography-tandem mass spectrometry (LC-MS/MS). We successfully analyzed 70 human gastric juice samples from patients with chronic superficial gastritis (CSG, n = 20), intestinal metaplasia (IM, n = 12), and gastric cancer (n = 38). Furthermore, we investigated the relevance between BA metabolism and gastric cancer. There were statistical differences in the metabolism of cholic acid (CA) into deoxycholic acid (DCA) based on the progression of CSG into IM and gastric cancer. Hence, the progression of gastric cancer might be related to the alterations in gut microbiome composition. We provide insight into the etiological mechanisms of the progression of gastric cancer and biomarkers to diagnose and treat gastric cancer.