Impact of Escherichia coli infection in broiler breeder chicks: The effect of oxidative stress on weight gain. (February 2020)

• Record Type: Journal Article
• Authors: da Rosa, Gilneia; Alba, Davi Fernando; Silva, Anielen D.; Gris, Anderson; Mendes, Ricardo E.; Mostardeiro, Vitor B.; Lopes, Thalison F.; Schetinger, Maria Rosa C.; Stefani, Lenita M.; Lopes, Marcos T.; Boiago, Marcel Manente; da Silva, Aleksandro S.
• Is Part Of: Microbial pathogenesis. Volume 139(2020)
• Publication Date: 2020-02
• Subjects: Colibacillosis -- Oxidative stress -- Performance -- Poultry farming; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2019.103861
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: The aim of this study was to determine whether oxidative stress occurs in Escherichia coli -infected broiler breeder chicks, as well as the impact of this infection on bird growth. Twenty birds, 25-day-old female birds were divided into two groups (n = 10 per group): an intraperitoneally-infected group (1 mL containing 1.5 × 10 8 CFU of E. coli ) and a control group that received 1 mL of culture medium (uninfected birds). Birds were weighed individually at the beginning and at the end of the experiment, and samples were collected on days 0, 5 and 10 post-infection (PI). No clinical signs were observed throughout the experimental period; nevertheless, on day 10 PI, there was lower growth and weight gain in infected birds than in the control group. The infected birds showed pericarditis and liver congestion, as well as moderate periportal inflammatory infiltrates with predominance of neutrophils. Significantly higher numbers of total leukocytes, lymphocytes, heterophils and monocytes were observed in the infected group on days 5 and 10 PI, as well as significantly higher total protein and globulin levels; albumin values significantly decreased over the same period. Levels of serum oxidative biomarkers (lipid peroxidation (TBARS) and free radicals (ROS)) were significantly higher at 10 PI, as was glutathione S-transferase (GST) activity during the same period. Hepatic ROS and protein thiol levels were significantly higher in E. coli -infected birds, as well as activities of the antioxidant enzymes catalase, superoxide dismutase. In the spleen, only GST activity was significantly higher for the infected group, unlike the brain, where SOD activity, ROS and non-protein thiol levels were significantly higher in infected birds than in the control group. These data suggested that colibacillosis causes oxidative stress in broiler breeder chicks, negatively affecting their weight gain. Highlights: Colibacillosis causes oxidative stress in broiler breeder chicks, negatively affecting health and growth. During E. coli infection, an increase in serum and tissue free radicals. Colibacillosis in broiler activate enzymatic and non-enzymatic antioxidant responses. E. coli infection reduce weight gain in birds.