Nasopharyngeal pneumococcal carriage in healthy Turkish children after 13-valent conjugated pneumococcal vaccine implementation in the national immunization program. Issue 2 (February 2020)

• Record Type: Journal Article
• Authors: Kanık Yüksek, Saliha; Tezer, Hasan; Gülhan, Belgin; Özkaya Parlakay, Aslınur; Güldemir, Dilek; Coskun-Ari, F. Filiz; Bedir Demirdağ, Tuğba; Kara Uzun, Aysun; Kızılgün, Murat; Solmaz, Sinem; Kılıç, Selçuk; Yalınay Çırak, Meltem; Baran Aksakal, Fatma Nur
• Is Part Of: Journal of infection and public health. Volume 13:Issue 2(2020)
• Page Start: 266
• Page End: 274
• Publication Date: 2020-02
• Subjects: ACIP advisory committee on immunization practices -- DNA deoxyribonucleic acid -- EDTA ethylene diamine tetraacetic acid -- IPD invasive pneumococcal disease -- mPCR multiplex polymerase chain reaction -- NIP national immunization program -- NMMRL national molecular microbiology reference laboratory -- NPC nasopharyngeal pneumococcal carriage -- PCR polymerase chain reaction -- PCV pneumococcal conjugated vaccines -- PPV polysaccharide pneumococcal vaccine -- RT-PCR real-time polymerase chain reaction -- Rxn reaction -- SPSS statistical package for the social sciences -- UK United Kingdom -- UV ultraviolet -- VS vaccine strains -- WHO World Health Organization; Pneumococcal conjugated vaccine -- PCV13 -- Nasopharyngeal carriage -- Healthy children -- Serotyping; Communicable diseases -- Periodicals; Public health -- Periodicals; Epidemiology -- Periodicals; Nosocomial infections -- Prevention -- Periodicals; Medical microbiology -- Periodicals; 614.4
• DOI: 10.1016/j.jiph.2019.10.009
• Languages: English
• ISSNs: 1876-0341

Abstract

Abstract: Background: In Turkey, pneumococcal conjugated vaccine (PCV) was introduced to the national immunization program as PCV7 in 2008, and was replaced with PCV13 in 2011. The aims of this study were to investigate the effects of PCV13 on nasopharyngeal pneumococcal carriage (NPC) by determining the serotype distribution, and to identify risk factors for carriage, in healthy Turkish children. Methods: This prospective study was conducted on 500 healthy children aged 0–13 years between April and November 2014. Nasopharyngeal swab samples were taken, and molecular method for capsular serotyping was performed by multiplex PCR. Results: Of 500 children, 43.4% were unvaccinated with a PCV (7- or 13-valent), 56.6% were vaccinated and The NPC rate was found to be 9.8%. Of 49 positive Streptococcus pneumoniae isolates, 26 (53%) were PCV13 vaccine strains (VSs), and 17 (34.7%) were non-VS. Six isolates (12.2%) were not typeable by the method applied. The most common serotypes detected were serotype 3 (18.3%), serotype 19F (14.2%), serotype 6A/B (8.1%), serotype 11A (8.1%), and serotype 15B (8.1%). The total coverage rate of the PCV13 serotypes was 60.4%. Conclusion: A significant decrease in carriage rate was detected within three years after the introduction of PCV13 in Turkey. However, the nasopharyngeal carriage of PCV13 strains was found to be interestingly high.