Evaluation of pre- and post-fusion Human metapneumovirus F proteins as subunit vaccine candidates in mice. Issue 9 (24th February 2020)

• Record Type: Journal Article
• Authors: Pilaev, Martin; Shen, Yao; Carbonneau, Julie; Venable, Marie-Christine; Rhéaume, Chantal; Lavigne, Sophie; Couture, Christian; Guarné, Alba; Hamelin, Marie-Ève; Boivin, Guy
• Is Part Of: Vaccine. Volume 38:Issue 9(2020)
• Page Start: 2122
• Page End: 2127
• Publication Date: 2020-02-24
• Subjects: Balb/C -- hMPV -- Fusion protein -- Pre-fusion -- Vaccine -- Immunisation; Vaccines -- Periodicals; 615.372
• DOI: 10.1016/j.vaccine.2020.01.047
• Languages: English
• ISSNs: 0264-410X

Abstract

Abstract: Human metapneumovirus (hMPV) is an important respiratory pathogen especially in young children and elderly subjects. Our objective was to assess the immunogenicity and protection conferred by predominant pre- and post-fusion (F) hMPV-F constructs in Balb/C mice. Immunizations without adjuvant were not immunogenic whereas alum-adjuvanted hMPV-F proteins, regardless of their conformations, generated comparable neutralizing antibody titers with undetectable pulmonary viral titers following viral challenge. In conclusion, we found no apparent advantage for mixtures of predominant pre-fusion F proteins over post-fusion conformations for hMPV vaccination in opposite to recent data obtained with the human respiratory syncytial virus.