Development of an immunochromatographic strip test for the rapid detection of soybean Bowman-Birk inhibitor. Issue 1 (1st January 2019)

• Record Type: Journal Article
• Authors: Wang, Yao; Li, Yanhong; WU, Jiabei; Pei, Yafeng; Chen, Xiujin; Sun, Yaning; Hu, Mei; Xing, Yunrui; Cao, Jinbo; Li, Zhaozhou; Fei, Peng; Deng, Ruiguang; Gu, Shaobin; Hu, Xiaofei
• Is Part Of: Food and agricultural immunology. Volume 30:Issue 1(2019)
• Page Start: 1202
• Page End: 1211
• Publication Date: 2019-01-01
• Subjects: Bowman-Birk inhibitor -- monoclonal antibody -- immunochromatographic strip; Immunochemistry -- Periodicals; Food -- Analysis -- Periodicals; Immunoassay -- Periodicals; 616.079
• DOI: 10.1080/09540105.2019.1680613
• Languages: English
• ISSNs: 0954-0105

Abstract

ABSTRACT: Bowman-Birk inhibitor (BBI) is the main type of soybean trypsin inhibitor, which is a kind of antinutritional factor. It can cause indigestion and hinder growth and development of human and animal. Herein, a rapid immunochromatographic strip was developed in a sandwich format using the anti-BBI monoclonal antibody (mAb) in order to specifically detect BBI. The mAbs showed high affinity towards BBI and no cross-reactivity with other soybean allergenic proteins and antinutritional factors. The assay could be performed within 10 min. The limit of detection of the test strip was 0.5 µg/mL for visual and 0.23 µg/mL for strip reader. The assay showed high specificity for BBI and recovery efficacy of BBI in spiked milk samples ranged between 83.24% and 89.86% with the coefficient of variation of less than 7.98%. Therefore, the test strip could be used as a rapid and reliable detection method for BBI qualitative or quantitative assessment.