Simultaneous detection of carbofuran and 3-hydroxy-carbofuran in vegetables and fruits by broad-specific monoclonal antibody-based ELISA. Issue 1 (1st January 2019)

• Record Type: Journal Article
• Authors: Lan, Jianqiang; Wang, Mifang; Ding, Shang; Fan, Yongmei; Diao, Xiaoping; Li, Qing X.; Zhao, Hongwei
• Is Part Of: Food and agricultural immunology. Volume 30:Issue 1(2019)
• Page Start: 1085
• Page End: 1096
• Publication Date: 2019-01-01
• Subjects: Carbofuran -- 3-hydroxycarbofuran -- icELISA -- immunoassay -- broad-specific monoclonal antibody; Immunochemistry -- Periodicals; Food -- Analysis -- Periodicals; Immunoassay -- Periodicals; 616.079
• DOI: 10.1080/09540105.2019.1664997
• Languages: English
• ISSNs: 0954-0105

Abstract

ABSTRACT: Carbofuran is a highly toxic insecticide and has been banned or restricted for pest control in many countries. Being a potent acetylcholinesterase inhibitor, carbofuran and its metabolite 3-hydroxycarbofuran (3-OH-CBF) are required for the analysis of their residues in food. However, an immunochemical method for simultaneous analysis of carbofuran and 3-OH-CBF is still not available. Herein, we report an enzyme-linked immunosorbent assay (ELISA) based on a broad-specific monoclonal antibody (mAb) for simultaneous detection of carbofuran and 3-OH-CBF. The mAb, designated as 2E3, against both carbofuran and 3-OH-CBF was used to develop an indirect competitive ELISA (icELISA) with 50% inhibition concentrations of 0.76 and 0.69 ng/mL, respectively. The developed icELISA was validated by UPLC-MS/MS and is suitable for the rapid and simultaneous detection of carbofuran and 3-OH-CBF in fruits and vegetables.