Aggregation-induced emission-based competitive lateral flow immunoassay for rapid detection of sulfamethazine in honey. Issue 1 (1st January 2019)

• Record Type: Journal Article
• Authors: Wang, Zexiang; Hu, Song; Zhang, Ganggang; Liu, Jintao; Xia, Jun; Peng, Juan; Lai, Weihua
• Is Part Of: Food and agricultural immunology. Volume 30:Issue 1(2019)
• Page Start: 1303
• Page End: 1317
• Publication Date: 2019-01-01
• Subjects: Lateral flow immunoassay -- competitive format -- aggregation-induced emission -- sulfonamides -- honey; Immunochemistry -- Periodicals; Food -- Analysis -- Periodicals; Immunoassay -- Periodicals; 616.079
• DOI: 10.1080/09540105.2019.1689929
• Languages: English
• ISSNs: 0954-0105

Abstract

ABSTRACT: Sulfamethazine (SMZ), widely used for disease prevention of bees, is harmful to consumers when excess SMZ residues are present in honey. In this study, a novel competitive lateral flow immunoassay (LFIA) was established by using aggregation-induced emission fluorescent microsphere (AIEFM) as label for the detection of SMZ. Results indicated that the limit of detection (LOD) for the developed competitive AIEFM-LFIA in honey was 0.028 ng/mL, and the corresponding linear range was 0.05−5 ng/mL. Recovery experiments showed that the average recovery range was 75.6–95.1% with coefficient variations of 5.7–12.9% in honey. The developed competitive AIEFM-LFIA was compatible with HPLC-MS/MS in detecting SMZ in 15 real samples from honey. In general, the proposed AIEFM-LFIA is well suited for the competitive format and has great potential for the detection of other small targets with single antigenic site in practical application. GRAPHICAL ABSTRACT: