Human chromatin remodeler cofactor, RNA interactor, eraser and writer sperm RNAs responding to obesity. Issue 1 (1st February 2020)

• Record Type: Journal Article
• Authors: Swanson, Grace M.; Estill, Molly; Diamond, Michael P.; Legro, Richard S.; Coutifaris, Christos; Barnhart, Kurt T.; Huang, Hao; Hansen, Karl R.; Trussell, J. C.; Coward, R. Matthew; Zhang, Heping; Goodrich, Robert; Krawetz, Stephen A.
• Is Part Of: Epigenetics. Volume 15:Issue 1/2(2020)
• Page Start: 32
• Page End: 46
• Publication Date: 2020-02-01
• Subjects: BMI -- sperm RNA -- obesity -- CREWs - chromatin remodeler cofactor RNA interactor eraser and writer RNAs; Epigenesis -- Periodicals; Epigenetica; 572.86505
• DOI: 10.1080/15592294.2019.1644880
• Languages: English
• ISSNs: 1559-2294

Abstract

ABSTRACT: In the United States almost 33% of adults are considered obese (BMI > 30 kg/m 2 ). Both animal models and to a lesser extent human studies, have associated BMI, a measure of obesity, with alterations in sperm DNA methylation and RNAs. Sperm RNAs from the Assessment of Multiple Gestations from Ovarian Stimulation trial, were isolated and sequenced. A Generalized Linear Model identified 487 BMI associated human sperm RNA elements (short exon-sized sequences). They partitioned into four patterns; a continual increase with BMI, increase once obese (BMI>30 kg/m 2 ); a steady decrease with BMI; and decrease once overweight (BMI 25 - 30 kg/m 2 ). Gene Ontology revealed a unique relationship between BMI and transcripts associated with chromosome organization, adipogenesis, cellular stress and obesity-related inflammation. Coregulatory networks linked by Chromatin remodeler cofactors, RNA interactors, Erasers and Writers (CREWs) were uncovered to reveal a hierarchical epigenetic response pathway.