Efficient separation of l-phosphinothricin from enzymatic reaction solution using cation-exchange resin. Issue 4 (3rd March 2020)

• Record Type: Journal Article
• Authors: Lv, Sheng-Zhi; Guo, Yu-Xing; Xue, Ya-Ping; Xu, Jian-Miao; Zheng, Yu-Guo
• Is Part Of: Separation science and technology. Volume 55:Issue 4(2020)
• Page Start: 779
• Page End: 787
• Publication Date: 2020-03-03
• Subjects: l-Phosphinothricin -- cation-exchange resin -- adsorption isotherms -- adsorption kinetics; Separation (Technology) -- Periodicals; 660.284205
• DOI: 10.1080/01496395.2019.1574824
• Languages: English
• ISSNs: 0149-6395

Abstract

ABSTRACT: The separation of l -phosphinothricin from reaction solution using cation-exchange resin was investigated. The adsorption and desorption capacities of seven different resins were first tested, and resin 001 × 7 was selected as the suitable resin. The equilibrium adsorption data followed the Freundlich isotherm model, and the adsorption kinetic data fitted with pseudo-second-order equation well. The effect of solution pH on the adsorption and the breakthrough/desorption curves of resin 001 × 7 was studied. The results indicated that under the optimized separation conditions (solution pH value 2.0, feed rate, 1.0 BV h −1, and desorption flow rate 0.5 BV h −1 ), the recovery ratio of l -phosphinothricin from reaction solution reached 98%.